{"product_id":"cst-39375s","title":"CST,  39375S, p21 Waf1\/Cip1 (F7S9M) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying p21 Waf1\/Cip1 mouse. Validated for Western Blotting,Immunoprecipitation,Immunohistochemistry (Paraffin). Available in 2 sizes. Highly specific and rigorously validated in-house, p21 Waf1\/Cip1 (F7S9M) Rabbit Monoclonal Antibody (CST #39375) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nImmunoprecipitation: 1:100\nImmunohistochemistry (Paraffin): 1:400 - 1:1600\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation, Immunohistochemistry (Paraffin)\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\np21 Waf1\/Cip1 (F7S9M) Rabbit Monoclonal Antibody recognizes endogenous levels of total p21 Waf1\/Cip1 protein. This antibody detects approximately 15 kDa and 105 kDa proteins of unknown identity in some cell lines.\nSpecies Reactivity: Mouse\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro11 of mouse p21 Waf1\/Cip1 protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nThe tumor suppressor protein p21 Waf1\/Cip1 acts as an inhibitor of cell cycle progression. It functions in stoichiometric relationships forming heterotrimeric complexes with cyclins and cyclin-dependent kinases. In association with CDK2 complexes, it serves to inhibit kinase activity and block progression through G1\/S (1). However, p21 may also enhance assembly and activity in complexes of CDK4 or CDK6 and cyclin D (2). The carboxy-terminal region of p21 is sufficient to bind and inhibit PCNA, a subunit of DNA polymerase, and may coordinate DNA replication with cell cycle progression (3). Upon UV damage or during cell cycle stages when cdc2\/cyclin B or CDK2\/cyclin A are active, p53 is phosphorylated and upregulates p21 transcription via a p53-responsive element (4). Protein levels of p21 are downregulated through ubiquitination and proteasomal degradation (5).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nCAP; CAP20; CDK; CDK-interacting protein 1; CDKI; Cdkn; Cdkn1; Cdkn1a; CDN1A; Cip1; Cyclin-dependent kinase inhibitor 1; cyclin-dependent kinase inhibitor 1A (P21); mda; mda6; Melanoma differentiation-associated protein; p21; p21\u003cwaf1\u003e; p21C; p21Cip1; p21W; p21WAF; SDI1; Waf; Waf1\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: M\nSENSITIVITY: Endogenous\nMW (kDa): 21\nSource\/Isotype: Rabbit IgG\u003c\/waf1\u003e","brand":"CST","offers":[{"title":"Default Title","offer_id":46800094134441,"sku":"39375S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-39375s","provider":"Iright","version":"1.0","type":"link"}