{"product_id":"cst-3945s","title":"CST,  3945S, Phospho-S6 Ribosomal Protein (Ser235\/236) (D57.2.2E) Rabbit Monoclonal Antibody (Biotinylated)","description":"Monoclonal Antibody for studying S6 Ribosomal Protein (Ser235\/Ser236) phosphate. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, Phospho-S6 Ribosomal Protein (Ser235\/236) (D57.2.2E) Rabbit Monoclonal Antibody (Biotinylated) (CST #3945) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 140 mM NaCl, 3 mM KCI, 10 mM sodium phosphate (pH 7.4) dibasic, 2 mM potassium phosphate monobasic, 2 mg\/mL BSA, and 50% glycerol. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nPhospho-S6 Ribosomal Protein (Ser235\/236) (D57.2.2E) Rabbit Monoclonal Antibody (Biotinylated) detects endogenous levels of ribosomal protein S6 only when phosphorylated at Ser235 and 236.\nSpecies Reactivity: Human, Mouse, Rat, Monkey, Mink, S. cerevisiae\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser235 and Ser236 of human ribosomal protein S6.\n\u003cb\u003eBackground\u003c\/b\u003e\nOne way that growth factors and mitogens effectively promote sustained cell growth and proliferation is by upregulating mRNA translation (1,2). Growth factors and mitogens induce the activation of p70 S6 kinase and the subsequent phosphorylation of S6 ribosomal protein. Phosphorylation of S6 ribosomal protein correlates with an increase in translation of mRNA transcripts that contain an oligopyrimidine tract in their 5' untranslated regions (2). These particular mRNA transcripts (5'TOP) encode proteins involved in cell cycle progression, as well as ribosomal proteins and elongation factors necessary for translation (2,3). Important S6 ribosomal protein phosphorylation sites include several residues (Ser235, Ser236, Ser240, and Ser244) located within a small, carboxy-terminal region of S6 protein (4,5).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\n40S ribosomal protein S6; Phosphoprotein NP33; ribosomal protein S6; RPS6; RS6; S6; S6 ribosomal protein; Small ribosomal subunit protein eS6\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R Mk Mi Sc\nSENSITIVITY: Endogenous\nMW (kDa): 32\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46800392388777,"sku":"3945S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-3945s","provider":"Iright","version":"1.0","type":"link"}