{"product_id":"cst-39475t","title":"CST,  39475T, IL-2R alpha\/CD25 (E9W2J) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying IL2RA mouse. Validated for WB,IP,IHC,IHC,IF,F,F. Available in 2 sizes. Highly specific and rigorously validated in-house, IL-2R alpha\/CD25 (E9W2J) Rabbit Monoclonal Antibody (CST #39475) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nImmunoprecipitation: 1:50\nIHC Leica Bond: 1:50 - 1:200\nImmunohistochemistry (Paraffin): 1:50 - 1:200\nImmunofluorescence (Frozen): 1:800\nFlow Cytometry (Fixed\/Permeabilized): 1:100 - 1:400\nFlow Cytometry (Live): 1:100 - 1:400\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier-free (BSA and azide free) version of this product see product # 95742 .\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation, IHC Leica Bond, Immunohistochemistry (Paraffin), Immunofluorescence (Frozen), Flow Cytometry (Fixed\/Permeabilized), Flow Cytometry (Live)\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nIL-2R alpha\/CD25 (E9W2J) Rabbit Monoclonal Antibody recognizes endogenous levels of total IL-2Rα\/CD25 protein. This antibody non-specifically labels myenteric nerves in the gut.\nSpecies Reactivity: Mouse\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of mouse IL-2Rα\/CD25 protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nInterleukin-2 (IL-2) is a T cell stimulatory cytokine best known for inducing T cell proliferation and NK cell proliferation and activation (1,2). IL-2 also promotes peripheral development of regulatory T cells (Tregs) (3,4). Conversely, IL-2 is involved in the activation-induced cell death (AICD) that is observed post T cell expansion by increasing levels of Fas on CD4 T cells (5). The effects of IL-2 are mediated through a trimeric receptor complex consisting of IL-2Rα, IL-2Rβ, and the common gamma chain, γc (1,2). IL-2Rα binds exclusively to IL-2 with low affinity and increases the binding affinity of the whole receptor complex including IL-2Rβ and γc subunits. IL-15 also binds to IL-2Rβ (1,2). γc is used by other cytokines, including IL-4, IL-7, IL-9, IL-15, and IL-21 (1,2). Binding of IL-2 initiates signaling cascades involving Jak1, Jak3, Stat5, and the PI3K\/Akt pathways (1,2).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nCD25; IL-2 receptor subunit alpha; IL-2-RA; IL-2R alpha chain; IL-2R subunit alpha; IL2-RA; Il2r; Il2ra; interleukin 2 receptor, alpha chain; Interleukin-2 receptor subunit alpha; Ly-4; Ly-43; MGC130561; OTTMUSP00000012002; p55; p55 chain\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: M\nSENSITIVITY: Endogenous\nMW (kDa): 40-80\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46800094036137,"sku":"39475T","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-39475t","provider":"Iright","version":"1.0","type":"link"}