{"product_id":"cst-3972s","title":"CST,  3972S, GRB2 Antibody","description":"Polyclonal Antibody for studying Grb2. Validated for Western Blotting. Highly specific and rigorously validated in-house, GRB2 Antibody (CST #3972) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nGRB2 Antibody detects endogenous levels of total GRB2 protein.\nSpecies Reactivity: Human, Mouse, Rat, Hamster, Monkey, Bovine\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nPolyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to carboxy-terminal residues of human GRB2. Antibodies are purified by protein A and peptide affinity chromatography.\n\u003cb\u003eBackground\u003c\/b\u003e\nGrowth factor receptor-binding protein 2 (GRB2) is an adaptor protein that is involved in RTK signal transduction. The SH2 domain of GRB2 binds to tyrosine phosphorylated proteins, such as EGFR, IRS-1, Shc, and Gab1 (1). The SH3 domain of GRB2 associates with SOS, which stimulates the Ras GTP-binding activity, leading to the activation of the MAP kinase and other signaling pathways. Phosphorylation of GRB2 at Tyr209 by Bcr-Abl and EGFR abolishes its association with SOS and negatively regulates downstream signaling (2).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nabundant SRC homology; Adapter protein GRB2; ASH; EGFRBP-GRB2; epidermal growth factor receptor-binding protein GRB2; epididymis secretory sperm binding protein; GRB2; Grb3-3; growth factor receptor bound protein 2; Growth factor receptor-bound protein 2; growth factor receptor-bound protein 3; HT027; MST084; MSTP084; NCKAP2; Protein Ash; SH2\/SH3 adapter GRB2\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R Hm Mk B\nSENSITIVITY: Endogenous\nMW (kDa): 25\nSOURCE: Rabbit","brand":"CST","offers":[{"title":"Default Title","offer_id":46799472754857,"sku":"3972S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-3972s","provider":"Iright","version":"1.0","type":"link"}