{"product_id":"cst-39754l","title":"CST,  39754L, Gasdermin D (E9S1X) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying gasdermin D mouse. Validated for Western Blotting,Immunoprecipitation. Available in 3 sizes. Highly specific and rigorously validated in-house, Gasdermin D (E9S1X) Rabbit Monoclonal Antibody (CST #39754) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nImmunoprecipitation: 1:50\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nGasdermin D (E9S1X) Rabbit Monoclonal Antibody recognizes endogenous levels of total Gasdermin D protein. This antibody recognizes the 30 kDa amino terminal fragment produced during pyroptosis by caspase-1.\nSpecies Reactivity: Human, Mouse, Rat, Hamster\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu60 of mouse Gasdermin D protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nGasdermin D (GSDMD), a member of the gasdermin family that includes GSDMA, GSDMB, and GSDMC, has been reported to have a critical role as a downstream effector of pyroptosis (1,2). Pyroptosis is a lytic type of cell death triggered by inflammasomes, multiprotein complexes assembled in response to pathogen-associated molecular patterns (PAMPs) or danger-associated molecular patterns (DAMPs) that result in the activation of caspase-1 and subsequent cleavage of pro-inflammatory cytokines IL-1β and IL-18 (3). Gasdermin D was identified by two independent groups as a substrate of inflammatory caspases, caspase-1 and caspase-11\/4\/5, producing two fragments: GSDMD-N and GSDMD-C. Cleavage results in release of an intramolecular inhibitory interaction between the N- and C-terminal domains, allowing the N-terminal fragment GSDMD-N to initiate pyroptosis through the formation of pores on the plasma membrane (4-7).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\n1810036L03Rik; AW558049; DF5; DF5L; Dfn; Dfna5l; gasdermin D; gasdermin domain containing 1; Gasdermin domain-containing protein 1; Gasdermin-D; Gasdermin-D, C-terminal; Gasdermin-D, N-terminal; gasderminD-1; Gsdmd; GsdmD-1; GSDMD-CT; GSDMD-NT; Gsdmdc; Gsdmdc1; M2-4\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R Hm\nSENSITIVITY: Endogenous\nMW (kDa): 53, 30\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46799473017001,"sku":"39754L","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-39754l","provider":"Iright","version":"1.0","type":"link"}