{"product_id":"cst-39862s","title":"CST,  39862S, CRAD Antibody","description":"Polyclonal Antibody for studying CRAD. Validated for Western Blotting,Immunoprecipitation. Highly specific and rigorously validated in-house, CRAD Antibody (CST #39862) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nImmunoprecipitation: 1:50\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nCRAD Antibody recognizes endogenous levels of total CRAD protein.\nSpecies Reactivity: Human\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nPolyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro200 of human CRAD protein. Antibodies are purified by peptide affinity chromatography.\n\u003cb\u003eBackground\u003c\/b\u003e\nCancer-related regulator of actin dynamics (CRAD, also CRACD, KIAA1211) regulates actin polymerization and epithelial integrity by sequestering actin capping proteins CAPZA and CAPZB. CRAD stabilizes the β-catenin\/E-cadherin complex. Loss of CRAD allows β-catenin to activate wnt signaling (1). While CRAD was identified as a tumor suppressor downregulated in colorectal cancer, its expression is upregulated in other human cancers, including non-small cell lung cancer (NSCLC) and breast cancer (1-3). In NSCLC cells, downregulation of CRAD inhibits proliferation and increases the rate of apoptosis (2). Mutation of CRAD in small cell lung cancer (SCLC) may serve as a prognostic marker for overall survival (4).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nCancer-related regulator of actin dynamics; Capping protein inhibiting regulator of actin dynamics; capping protein inhibiting regulator of actin dynamicsp; Capping protein-inhibiting regulator of actin dynamics; CRACD; CRAD; DKFZp564H1363; KIAA1211; RP11-646I6.2\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H\nSENSITIVITY: Endogenous\nMW (kDa): 200\nSOURCE: Rabbit","brand":"CST","offers":[{"title":"Default Title","offer_id":46799474032809,"sku":"39862S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-39862s","provider":"Iright","version":"1.0","type":"link"}