{"product_id":"cst-40354t","title":"CST,  40354T, Nectin-3\/PVRL3 (F9R9R) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying PVRL3. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, Nectin-3\/PVRL3 (F9R9R) Rabbit Monoclonal Antibody (CST #40354) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nImmunoprecipitation: 1:50\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nNectin-3\/PVRL3 (F9R9R) Rabbit Monoclonal Antibody recognizes endogenous levels of total nectin-3\/PVRL3 protein.\nSpecies Reactivity: Human\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val521 of human nectin-3\/PVRL3 protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nNectin cell adhesion molecule 3 (Nectin-3), also known as poliovirus receptor-related protein 3 (PVRL3) and CD113, is a member of the immunoglobulin-like cellular adhesion molecule family and plays an important role in calcium-independent cellular adhesion (1,2). There are four nectin family members, nectin-1, nectin-2, nectin-3, and nectin-4. They are composed of an ectodomain containing three tandem Ig-like regions arranged as an N-terminal variable-like domain followed by two constant-like domains, a single transmembrane region, and a cytoplasmic domain that binds to the actin cytoskeleton through the adaptor protein afadin (3,4). Nectin-3 can engage in homophilic, as well as heterophilic cell adhesion by engaging with other nectin family members (5). In the nervous system, nectin-3 expression is associated with chronic stress-induced synaptic abnormalities and has been proposed as a target for stress-related cognitive disorder treatment (6,7). Nectin-3 can also interact with the immune system. Nectin-3 expressed on antigen-presenting cells (APCs) and tumor cells can act as a ligand for the inhibitory receptor TIGIT, expressed on activated T cell subsets and natural killer (NK) cells (8). Aberrant expression of nectin-3 has been observed in multiple cancers, including breast, pancreatic, lung, colorectal, and ovarian cancer (9-14).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nCD113; CDw113; DKFZp566B0846; FLJ90624; NECT3; nectin 3; Nectin cell adhesion molecule 3; Nectin-3; NECTIN3; poliovirus receptor-related 3; Poliovirus receptor-related protein 3; PPR3; PRR3; PVRL3; PVRR3\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H\nSENSITIVITY: Endogenous\nMW (kDa): 80-100\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46799477211305,"sku":"40354T","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-40354t","provider":"Iright","version":"1.0","type":"link"}