{"product_id":"cst-40364t","title":"CST,  40364T, HMGCS2 (D8B5M) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying HMGCS2. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, HMGCS2 (D8B5M) Rabbit Monoclonal Antibody (CST #40364) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nHMGCS2 (D8B5M) Rabbit Monoclonal Antibody recognizes endogenous levels of total HMGCS2 protein. This antibody does not cross-react with HMGCS1 protein.\nSpecies Reactivity: Human, Mouse, Rat\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala45 of human HMGCS2 protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nMitochondrial 3-hydroxy-3-methylglutaryl-CoA synthase (HMGCS2) generates hydroxymethylglutaryl-CoA (HMG-CoA) from acetyl-CoA and acetoacetyl-CoA, a rate-limiting step in ketogenesis (1). Starvation or a high-fat and low-carbohydrate diet increases the levels of hepatic FGF21, which in turn up-regulates HMGCS2 expression (2). Furthermore, mTORC1 inhibition was shown to be required for the increase of HMGCS2 expression mediated by PPARα in response to fasting (3). In addition, studies on mice lacking HMGCS2 suggest that ketogenesis plays a role in the prevention of diet-induced fatty liver injury and hyperglycemia (4).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\n3-hydroxy-3-methylglutaryl coenzyme A synthase; 3-hydroxy-3-methylglutaryl-CoA synthase 2; 3-hydroxy-3-methylglutaryl-CoA synthase 2 (mitochondrial); 3-hydroxy-3-methylglutaryl-Coenzyme A synthase 2 (mitochondrial); HMCS2; HMG-CoA synthase; HMGCS2; Hydroxymethylglutaryl-CoA synthase, mitochondrial; mitochondrial 3-hydroxy-3-methylglutaryl-CoA synthase; testicular tissue protein Li 88\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R\nSENSITIVITY: Endogenous\nMW (kDa): 50\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46799477473449,"sku":"40364T","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-40364t","provider":"Iright","version":"1.0","type":"link"}