{"product_id":"cst-40485s","title":"CST,  40485S, SARS-CoV-2 Nsp2 Antibody","description":"Polyclonal Antibody for studying nsp2-pp1a-pp1ab SARS-CoV-2. Validated for Western Blotting. Highly specific and rigorously validated in-house, SARS-CoV-2 Nsp2 Antibody (CST #40485) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/mL BSA, and 50% glycerol. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nSARS-CoV-2 Nsp2 Antibody recognizes endogenous levels of total SARS-CoV-2 Nsp2 protein in infected cells.\nSpecies Reactivity: Virus\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nPolyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of SARS-CoV-2 Nsp2 protein. Antibodies are purified by peptide affinity chromatography.\n\u003cb\u003eBackground\u003c\/b\u003e\nThe cause of the COVID-19 pandemic is a novel and highly pathogenic coronavirus, termed SARS-CoV-2 (severe acute respiratory syndrome coronavirus-2). SARS-CoV-2 is a member of the Coronaviridae family of viruses (1). The genome of SARS-CoV-2 is relatively large and encodes up to 29 open reading frames (ORFs). These include ORF1a and ORF1b (further processed into 16 non-structural proteins), nine accessory proteins, and four canonical structural proteins: spike (S), envelope (E), membrane (M), and nucleocapsid (N) (2). Nsp2 (non-structural protein 2) is derived from ORF1a and ORF1b (2). It is reported to interact with a protein involved in translational repression known as GIGYF2. GIGYF2 normally interacts with another translational repressor, 4EHP. These proteins together in a larger complex function to inhibit translation of mRNAs that may be defective during development. Upon infection with SARS-CoV-2, however, Nsp2 binds to GIGYF2 and exerts a stronger effect on host cell translation by 4EHP dependent and independent mechanisms, with inhibition of interferon translation being one of the notable effects (3).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nnon-structural protein 2; non-structural protein 2 (181-818); nsp2; nsp2-pp1a-pp1ab; p65 homolog; SARS-CoV-2 nsp2\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: Vir\nSENSITIVITY: Endogenous\nMW (kDa): 80\nSOURCE: Rabbit","brand":"CST","offers":[{"title":"Default Title","offer_id":46800243327145,"sku":"40485S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-40485s","provider":"Iright","version":"1.0","type":"link"}