{"product_id":"cst-40491t","title":"CST,  40491T, M-Cadherin (D4B9L) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying CDH15 mouse. Validated for WB,IP,IF,IF. Available in 2 sizes. Highly specific and rigorously validated in-house, M-Cadherin (D4B9L) Rabbit Monoclonal Antibody (CST #40491) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nImmunoprecipitation: 1:200\nImmunofluorescence (Frozen): 1:200\nImmunofluorescence (Immunocytochemistry): 1:200\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 99890 .\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Frozen), Immunofluorescence (Immunocytochemistry)\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nM-Cadherin (D4B9L) Rabbit Monoclonal Antibody recognizes endogenous levels of total M-Cadherin protein. The lower band in the doublet shown in C2C12 and RD extracts is unglycosylated M-Cadherin. The identity of the ~78 kDa band that is present in the C2C12 extract is not known but is likely to be a breakdown product in the lysate as it is absent in the IP input and pull-down. Note that human M-Cadherin runs slightly lower than mouse M-Cadherin.\nSpecies Reactivity: Human, Mouse\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Alanine 399 of human M-Cadherin protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nCadherins are a superfamily of transmembrane glycoproteins that contain cadherin repeats of approximately 100 residues in their extracellular domain. Cadherins mediate calcium-dependent cell-cell adhesion and play critical roles in normal tissue development (1). The classic cadherin subfamily includes N-, P-, R-, B-, and E-cadherins, as well as about ten other members that are found in adherens junctions, a cellular structure near the apical surface of polarized epithelial cells. The cytoplasmic domain of classical cadherins interacts with β-catenin, γ-catenin (also called plakoglobin), and p120 catenin. β-catenin and γ-catenin associate with α-catenin, which links the cadherin-catenin complex to the actin cytoskeleton (1,2). While β- and γ-catenin play structural roles in the junctional complex, p120 regulates cadherin adhesive activity and trafficking (1-4). Investigators consider E-cadherin an active suppressor of invasion and growth of many epithelial cancers (1-3). Research studies indicate that cancer cells have upregulated N-cadherin in addition to loss of E-cadherin. This change in cadherin expression is called the \"cadherin switch.\" N-cadherin cooperates with the FGF receptor, leading to overexpression of MMP-9 and cellular invasion (3). Research studies have shown that in endothelial cells, VE-cadherin signaling, expression, and localization correlate with vascular permeability and tumor angiogenesis (5,6). Investigators have also demonstrated that expression of P-cadherin, which is normally present in epithelial cells, is also altered in ovarian and other human cancers (7,8). M-cadherin is a cell-cell adhesion molecule expressed in satellite cells, a collection of adult stem cells\/myogenic progenitors found in mature muscle tissue (9). Research studies indicate that M-cadherin may be involved in the recognition and fusion of adjacent cells, and may play an important role in activating satellite cell division (10).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nAI323380; CAD15; cadherin 15; Cadherin-14; Cadherin-15; Cdh14; Cdh15; M cadherin; M-cadherin; Mca; Mcad; Muscle cadherin\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M\nSENSITIVITY: Endogenous\nMW (kDa): 130\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46799478849705,"sku":"40491T","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-40491t","provider":"Iright","version":"1.0","type":"link"}