{"product_id":"cst-4073t","title":"CST,  4073T, Pan-Cadherin (28E12) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying N-Cadherin\/P-Cadherin\/E-Cadherin\/R-cadherin. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, Pan-Cadherin (28E12) Rabbit Monoclonal Antibody (CST #4073) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nPan-Cadherin (28E12) Rabbit Monoclonal Antibody detects endogenous levels of total cadherin proteins and has a preference for N-, R-, and E-Cadherin. This antibody does not prefer P- or VE- Cadherin.\nSpecies Reactivity: Human, Mouse, Rat\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to a conserved region of human N-, R-, and, E-Cadherin.\n\u003cb\u003eBackground\u003c\/b\u003e\nCadherins are a superfamily of transmembrane glycoproteins that contain cadherin repeats of approximately 100 residues in their extracellular domain. Cadherins mediate calcium-dependent cell-cell adhesion and play critical roles in normal tissue development (1). The classic cadherin subfamily includes N-, P-, R-, B-, and E-cadherins, as well as about ten other members that are found in adherens junctions, a cellular structure near the apical surface of polarized epithelial cells. The cytoplasmic domain of classical cadherins interacts with β-catenin, γ-catenin (also called plakoglobin), and p120 catenin. β-catenin and γ-catenin associate with α-catenin, which links the cadherin-catenin complex to the actin cytoskeleton (1,2). While β- and γ-catenin play structural roles in the junctional complex, p120 regulates cadherin adhesive activity and trafficking (1-4). Investigators consider E-cadherin an active suppressor of invasion and growth of many epithelial cancers (1-3). Research studies indicate that cancer cells have upregulated N-cadherin in addition to loss of E-cadherin. This change in cadherin expression is called the \"cadherin switch.\" N-cadherin cooperates with the FGF receptor, leading to overexpression of MMP-9 and cellular invasion (3). Research studies have shown that in endothelial cells, VE-cadherin signaling, expression, and localization correlate with vascular permeability and tumor angiogenesis (5,6). Investigators have also demonstrated that expression of P-cadherin, which is normally present in epithelial cells, is also altered in ovarian and other human cancers (7,8).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nACOGS; Arc-1; ARVD14; BCDS1; CAD4; CADH1; CADH2; CADH3; CADH4; Cadherin; cadherin 1; cadherin 1, E-cadherin (epithelial); cadherin 1, type 1; cadherin 1, type 1, E-cadherin (epithelial); cadherin 2; cadherin 2, N-cadherin (neuronal); cadherin 2, type 1, N-cadherin (neuronal); cadherin 3; cadherin 3, type 1, P-cadherin (placental); cadherin 4; cadherin 4, type 1, preproprotein; cadherin 4, type 1, R-cadherin (retinal); Cadherin E; Cadherin-1; Cadherin-2; Cadherin-3; Cadherin-4; calcium-dependent adhesion protein, epithelial; calcium-dependent adhesion protein, neuronal; calcium-dependent adhesion protein, placental; CAM 120\/80; CD324; CD325; CDH1; CDH2; CDH3; CDH4; CDHE; CDHN; CDHP; CDw325; cell-CAM 120\/80; E-Cad\/CTF1; E-Cad\/CTF2; E-Cad\/CTF3; E-cadherin; E-cadherin 1; ECAD; epididymis secretory sperm binding protein; Epithelial cadherin; FLJ22202; FLJ40547; HJMD; LCAM; MGC126700; MGC138355; N Cadherin; N-cadherin; N-cadherin 1; NCAD; Neural cadherin; neural-cadherin; P-cadherin; PCAD; Placental cadherin; R-CAD; R-cadherin; RCAD; Retinal cadherin; UVO; Uvomorulin\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R\nSENSITIVITY: Endogenous\nMW (kDa): 130-150\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46799481503913,"sku":"4073T","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-4073t","provider":"Iright","version":"1.0","type":"link"}