{"product_id":"cst-40908sf","title":"CST,  40908SF, BCKDH-E1 alpha (E7Y1I) Rabbit Monoclonal Antibody (BSA and Azide Free)","description":"Monoclonal Antibody for studying BCKDH E1-alpha. Validated for ELISA+. Highly specific and rigorously validated in-house, BCKDH-E1 alpha (E7Y1I) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #40908) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nThis formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT\u0026amp;RUN or CUT\u0026amp;Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions\/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity.\n\u003cb\u003eFormulation\u003c\/b\u003e\nSupplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free.\n\u003cb\u003eStorage\u003c\/b\u003e\nStore at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze\/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance.\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nBCKDH-E1 alpha (E7Y1I) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total BCKDH-E1α protein.\nSpecies Reactivity: Human, Mouse, Rat\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to a fragment of human BCKDH-E1α protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nBranched-chain amino acids (BCAAs) leucine, isoleucine, and valine are essential amino acids in mammals, but elevated levels of BCAAs have been implicated in cardiovascular and metabolic disorders (1). The branched-chain α-keto acid dehydrogenase complex (BCKDH) catalyzes the rate-limiting step in the BCAA degradation pathway (2,3). Branched-chain α-keto acid decarboxylase (BCKDH-E1) is one of three enzymatic components in this complex (3). The α subunit of BCKDH-E1 (BCKDH-E1α) is critical for the regulation of BCKDH. Phosphorylation of BCKDH-E1α was shown to play a key role in regulating the enzymatic activity of this complex (3-5).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\n2-oxoisovalerate dehydrogenase (lipoamide); 2-oxoisovalerate dehydrogenase subunit alpha, mitochondrial; BCKDE1A; BCKDH E1-alpha; BCKDHA; branched chain keto acid dehydrogenase E1 alpha protein; branched chain keto acid dehydrogenase E1 subunit alpha; branched chain keto acid dehydrogenase E1, alpha polypeptide; Branched-chain alpha-keto acid dehydrogenase E1 component alpha chain; FLJ45695; MSU; MSUD1; ODBA; OVD1A\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R\nSENSITIVITY: Endogenous\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46800312434857,"sku":"40908SF","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-40908sf","provider":"Iright","version":"1.0","type":"link"}