{"product_id":"cst-4108s","title":"CST,  4108S, LC3A\/B Antibody","description":"Polyclonal Antibody for studying LC3A\/LC3B. Validated for Western Blotting,Immunofluorescence (Immunocytochemistry),Flow Cytometry (Fixed\/Permeabilized). Highly specific and rigorously validated in-house, LC3A\/B Antibody (CST #4108) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nImmunofluorescence (Immunocytochemistry): 1:100 - 1:400\nFlow Cytometry (Fixed\/Permeabilized): 1:50 - 1:100\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed\/Permeabilized)\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nLC3A\/B Antibody detects endogenous levels of total LC3A and LC3B proteins. Cross-reactivity may exist with LC3C. Stronger reactivity is observed with the type II form of LC3A\/B.\nSpecies Reactivity: Human, Mouse, Rat\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nPolyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly40 of LC3B. Antibodies are purified by protein A and peptide affinity chromatography.\n\u003cb\u003eBackground\u003c\/b\u003e\nAutophagy is a catabolic process for the autophagosomic-lysosomal degradation of bulk cytoplasmic contents (1,2). Autophagy is generally activated by conditions of nutrient deprivation, but it has also been associated with a number of physiological processes including development, differentiation, neurodegenerative diseases, infection, and cancer (3). Autophagy marker Light Chain 3 (LC3) was originally identified as a subunit of microtubule-associated proteins 1A and 1B (termed MAP1LC3) (4) and subsequently found to contain similarity to the yeast protein Apg8\/Aut7\/Cvt5 critical for autophagy (5). Three human LC3 isoforms (LC3A, LC3B, and LC3C) undergo posttranslational modifications during autophagy (6-9). Cleavage of LC3 at the carboxy terminus immediately following synthesis yields the cytosolic LC3-I form. During autophagy, LC3-I is converted to LC3-II through lipidation by a ubiquitin-like system involving Atg7 and Atg3 that allows for LC3 to become associated with autophagic vesicles (6-10). The presence of LC3 in autophagosomes and the conversion of LC3 to the lower migrating form, LC3-II, have been used as indicators of autophagy (11).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nATG8E; ATG8F; Autophagy-related protein LC3 A; Autophagy-related protein LC3 B; Autophagy-related ubiquitin-like modifier LC3 A; Autophagy-related ubiquitin-like modifier LC3 B; LC3; LC3A; LC3B; MAP1 light chain 3-like protein 1; MAP1 light chain 3-like protein 2; MAP1A\/1B light chain 3 A; MAP1A\/1BLC3; MAP1A\/MAP1B LC3 A; MAP1A\/MAP1B LC3 B; MAP1A\/MAP1B light chain 3 A; MAP1A\/MAP1B light chain 3 B; MAP1ALC3; MAP1BLC3; MAP1LC3; MAP1LC3A; MAP1LC3B; MAP1LC3B-a; microtubule associated protein 1 light chain 3 alpha; microtubule associated protein 1 light chain 3 beta; Microtubule-associated protein 1 light chain 3 alpha; Microtubule-associated protein 1 light chain 3 beta; microtubule-associated proteins 1A\/1B light chain 3; Microtubule-associated proteins 1A\/1B light chain 3A; Microtubule-associated proteins 1A\/1B light chain 3B; MLP3A; MLP3B\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R\nSENSITIVITY: Endogenous\nMW (kDa): 14, 16\nSOURCE: Rabbit","brand":"CST","offers":[{"title":"Default Title","offer_id":46799482912937,"sku":"4108S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-4108s","provider":"Iright","version":"1.0","type":"link"}