{"product_id":"cst-41228t","title":"CST,  41228T, HLA-F (F4B9F) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying HLA-F. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, HLA-F (F4B9F) Rabbit Monoclonal Antibody (CST #41228) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nHLA-F (F4B9F) Rabbit Monoclonal Antibody recognizes endogenous levels of total HLA-F protein.\nSpecies Reactivity: Human\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gln162 of human HLA-F protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nHLA class I histocompatibility antigen, alpha chain F (HLA-F) is a nonclassical member of the HLA class I family and has less genetic diversity than classical HLA molecules (1-4). HLA-F expression is primarily intracellular but has been observed on the surface in some contexts, including some activated immune cell subsets, virus-infected cells, invasive extravillous cytotrophoblasts, and various cancer types (5,6). HLA-F can be expressed on the surface as an open conformer monomer or dimer, or in a complex with β2-microglobulin and peptide. As an open conformer monomer, HLA-F is observed to interact with activating KIR3DS1 and inhibitory KIR3DL2 (7,8). The HLA-F\/β2-microglobulin peptide complex interacts with inhibitory receptors ILT2 and ILT4 (9,10). It is hypothesized that high surface expression of HLA-F in tumors can be a mechanism of immune escape through interaction with inhibitory receptors on immune cells (5). In the context of viral infection, HLA-F interaction with activating KIR3DS1 on natural killer (NK) cells can result in NK cell degranulation and cytokine production (7).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nCDA12; HLA class I histocompatibility antigen, alpha chain F; HLA class I molecule; HLA F antigen; HLA-5.4; HLA-CDA12; HLA-F; HLAF; Leukocyte antigen F; major histocompatibility complex, class I, F; MHC class I antigen; MHC class I antigen F; MHC class Ib antigen\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H\nSENSITIVITY: Endogenous\nMW (kDa): 42\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46799484321961,"sku":"41228T","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-41228t","provider":"Iright","version":"1.0","type":"link"}