{"product_id":"cst-4129s","title":"CST,  4129S, Cyclin E1 (HE12) Mouse Monoclonal Antibody","description":"Monoclonal Antibody for studying Cyclin E1. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, Cyclin E1 (HE12) Mouse Monoclonal Antibody (CST #4129) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 85718 .\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nCyclin E1 (HE12) Mouse Monoclonal Antibody detects endogenous levels of total cyclin E1 protein. This antibody does not cross react with cyclin E2.\nSpecies Reactivity: Human, Monkey\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a recombinant human cyclin E1 protein. The epitope has been mapped to residues surrounding Ile360.\n\u003cb\u003eBackground\u003c\/b\u003e\nCyclin E1 and cyclin E2 can associate with and activate CDK2 (1). Upon DNA damage, upregulation\/activation of the CDK inhibitors p21 Waf1\/Cip1 and p27 Kip1 prevent cyclin E\/CDK2 activation, resulting in G1\/S arrest. When conditions are favorable for cell cycle progression, cyclin D\/CDK4\/6 phosphorylates Rb and is thought to reduce the activity of p21 Waf1\/Cip1 and p27 Kip1, allowing subsequent activation of cyclin E\/CDK2 (1,2). Cyclin E\/CDK2 further phosphorylates Rb to allow progression into S-phase, where cyclin E\/CDK2 is thought to phosphorylate and activate multiple proteins involved in DNA synthesis (2,3). Turnover of cyclin E is largely controlled by phosphorylation that results in SCFFbw7-mediated ubiquitination and proteasome-dependent degradation (4,5). Cyclin E1 is phosphorylated at multiple sites including Thr62, Ser88, Ser72, Thr380, and Ser384, and is controlled by at least two kinases, CDK2 and GSK-3 (6,7).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nCCNE; CCNE1; cyclin E1; cyclin Es; cyclin Et; G1\/S-specific cyclin-E1; pCCNE1\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H Mk\nSENSITIVITY: Endogenous\nMW (kDa): 48\nSource\/Isotype: Mouse IgG1","brand":"CST","offers":[{"title":"Default Title","offer_id":46799484780713,"sku":"4129S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-4129s","provider":"Iright","version":"1.0","type":"link"}