{"product_id":"cst-4172s","title":"CST,  4172S, MKK7 Antibody","description":"Polyclonal Antibody for studying MKK7. Validated for Western Blotting,Immunoprecipitation. Highly specific and rigorously validated in-house, MKK7 Antibody (CST #4172) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nImmunoprecipitation: 1:50\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nMKK7 Antibody detects endogenous levels of total MKK7. This antibody does not cross-react with SEK1\/MKK4, MEK1\/2 or other MAP kinase kinases.\nSpecies Reactivity: Human, Mouse, Rat, Monkey\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nPolyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the amino-terminal residues of human MKK7. Antibodies are purified by protein A and peptide affinity chromatography.\n\u003cb\u003eBackground\u003c\/b\u003e\nMKK7 is a MAP kinase kinase that serves as a specific activator of the SAPK\/JNK pathway (1,2). MKK7 is strongly activated by TNF-α, as well as other environmental stresses, whereas SEK1\/MKK4, which activates both p38 and SAPK\/JNK pathways, is not activated by TNF-α (2). Sequence alignment of the activation loop of the MAP kinase kinase family members indicates that Ser271 and Thr275 are potential phosphorylation sites that are crucial for the kinase acivity.\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nc-Jun N-terminal kinase kinase 2; Dual specificity mitogen-activated protein kinase kinase 7; JNK kinase 2; JNK-activating kinase 2; JNKK 2; JNKK2; MAP kinase kinase 7; MAP2K7; MAPK\/ERK kinase 7; MAPKK 7; MAPKK7; MEK; MEK 7; MEK7; mitogen-activated protein kinase kinase 7; MKK7; MP2K7; PRKMK7; SAPK kinase 4; SAPKK-4; SAPKK4; SKK4; Stress-activated protein kinase kinase 4\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R Mk\nSENSITIVITY: Endogenous\nMW (kDa): 48\nSOURCE: Rabbit","brand":"CST","offers":[{"title":"Default Title","offer_id":46799490744489,"sku":"4172S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-4172s","provider":"Iright","version":"1.0","type":"link"}