{"product_id":"cst-4174s","title":"CST,  4174S, TPA (12-O-Tetradecanoylphorbol-13-Acetate)","description":"Chemical Modulators for studying in the research area.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nTPA is supplied as a 1 mg powder. Store at -20C. Before use, dissolve powder in 1 ml DMSO to yield a 1.6 mM stock solution. For working concentration of 200 μM, add 125 μl stock solution to 875 μl DMSO. Treat cells with 200 nM TPA for 20-30 minutes. TPA is soluble in DMSO and ethanol.\n\u003cb\u003eStorage\u003c\/b\u003e\nStore lyophilized or in solution at -20ºC, desiccated. Protect from light. In lyophilized form, the chemical is stable for 24 months. Once in solution, use within 3 months to prevent loss of potency. Aliquot to avoid multiple freeze\/thaw cycles.\n\u003cb\u003eBackground\u003c\/b\u003e\nTPA (12-O-tetradecanoylphorbol-13-acetate) \/ PMA (phorbol-12-myristate-13-acetate) is the most commonly used phorbol ester. It binds and activates protein kinase C, causing a wide range of effects in cells and tissues (1,2). TPA has been demonstrated to be a potent tumor promoter in mouse skin (3). Conversely, it has been shown to be an effective cancer therapeutic agent in myelocytic leukemia patients, and has been indicated as a potential colorectal cancer therapeutic (4-5). TPA has also been shown to increase white blood cell and neutrophil counts in solid tumor cancer patients (6).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nphorbol ester; PKC; PMA; protein kinase C","brand":"CST","offers":[{"title":"Default Title","offer_id":46800694968489,"sku":"4174S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-4174s","provider":"Iright","version":"1.0","type":"link"}