{"product_id":"cst-41968sf","title":"CST,  41968SF, MAdCAM-1 (E1V8F) Rabbit Monoclonal Antibody (BSA and Azide Free)","description":"Monoclonal Antibody for studying MADCAM1. Validated for Western Blotting,Immunohistochemistry (Paraffin). Highly specific and rigorously validated in-house, MAdCAM-1 (E1V8F) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #41968) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nThis product is the carrier free version of product #93757. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT\u0026amp;RUN or CUT\u0026amp;Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions\/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity.\n\u003cb\u003eFormulation\u003c\/b\u003e\nSupplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 93757\n\u003cb\u003eStorage\u003c\/b\u003e\nStore at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze\/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance.\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nMAdCAM-1 (E1V8F) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes transfected levels of total MAdCAM-1 protein by western blot and endogenous levels of total MAdCAM-1 protein by immunohistochemistry. The observed molecular weight of 60-85 kDa differs from the predicted molecular weight of 40 kDa due to glycosylation.\nSpecies Reactivity: Human\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human MAdCAM-1 protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nMucosal addressin cell adhesion molecule 1 (MAdCAM-1) is a glycoprotein expressed by endothelial cells at venules of Peyer's patches, intestinal lamina propria, and mesenteric lymph nodes (1). MAdCAM-1 contributes to lymphocyte homing to these tissues, serving as a ligand for α4β7, L-Selectin, and, to a lesser extent, α4β1 (2-6). Expression of MAdCAM-1 is upregulated in patients with inflammatory bowel disease, and blocking MAdCAM-1 reduces colon inflammation by disrupting lymphocyte homing to the intestinal tract (7). In ulcerative colitis, disease activity may be a result of the L-Selectin ligand carbohydrates attached to MAdCAM-1 by -acetylglucosamine-6- -sulfotransferase-1 (GlcNAc6ST-1) rather than the expression levels of MAdCAM-1 alone (4).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nhMAdCAM-1; MACAM1; MADCA; MAdCAM-1; MADCAM1; Mucosal addressin cell adhesion molecule 1; mucosal addressin cell adhesion molecule-1; mucosal vascular addressin cell adhesion molecule 1\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H\nSENSITIVITY: Endogenous (IHC-P), Transfected (W)\nMW (kDa): 60-85\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46799492743337,"sku":"41968SF","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-41968sf","provider":"Iright","version":"1.0","type":"link"}