{"product_id":"cst-42042s","title":"CST,  42042S, TMEM35 (F3G2T) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying TMEM35. Validated for Western Blotting,Simple Western™,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, TMEM35 (F3G2T) Rabbit Monoclonal Antibody (CST #42042) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nSimple Western™: 1:10 - 1:50\nImmunoprecipitation: 1:50\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nTMEM35 (F3G2T) Rabbit Monoclonal Antibody recognizes endogenous levels of total TMEM35 protein.\nSpecies Reactivity: Human, Mouse, Rat\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human TMEM35 protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nNicotinic acetylcholine receptors (nAChRs) are ligand-gated ion channels found throughout the peripheral and central nervous system (CNS), involved in fast synaptic transmission and a wide range of physiological functions (1). In mammals, neuronal nAChRs are classified through combinations of 11 alpha (α2-α7, α9, and α10) and beta (β2-β4) subunits, with the α7 and α4β2 subtypes being the most prevalent in the brain (2). Dysfunction of these receptors has been implicated in several neurological diseases, including Alzheimer's disease (AD), Parkinson's disease (PD), schizophrenia, and neuropathic pain (3-5). Transmembrane protein 35 (TMEM35), also called novel acetylcholine receptor chaperone (NACHO), is a molecular chaperone encoded by the gene. It is crucial for the proper assembly, surface expression, and function of several neuronal nAChR subtypes in the brain, especially the α7 subtype (6). CNS α7 nAChRs have been strongly implicated in AD and PD, making TMEM35 a potential therapeutic target and tool to better understand CNS nAChRs in neurodegeneration (3-5). TMEM35 has also been linked to the development and progression of osteosarcoma (OSA) by playing a role in OSA cell proliferation and migration (7).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nFLJ14084; NACHO; nAChR regulator; nicotinic acetylcholine receptor regulator; Novel acetylcholine receptor chaperone; TM35A; TMEM35; TMEM35A; TMM35; Transmembrane protein 35; Transmembrane protein 35A; TUF-1\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R\nSENSITIVITY: Endogenous\nMW (kDa): 18\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46799493497001,"sku":"42042S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-42042s","provider":"Iright","version":"1.0","type":"link"}