{"product_id":"cst-4249t","title":"CST,  4249T, PI3 Kinase p110 alpha (C73F8) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying PI3K p110 alpha. Validated for Western Blotting,Simple Western™,Immunoprecipitation. Available in 3 sizes. Highly specific and rigorously validated in-house, PI3 Kinase p110 alpha (C73F8) Rabbit Monoclonal Antibody (CST #4249) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nSimple Western™: 1:10 - 1:50\nImmunoprecipitation: 1:50\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nPI3 Kinase p110 alpha (C73F8) Rabbit Monoclonal Antibody detects endogenous levels of total PI3K p110α protein.\nSpecies Reactivity: Human, Mouse, Rat, Bovine\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide surrounding Asp520 of the sequence of human PI3K p110α.\n\u003cb\u003eBackground\u003c\/b\u003e\nPhosphoinositide 3-kinase (PI3K) catalyzes the production of phosphatidylinositol-3,4,5-triphosphate by phosphorylating phosphatidylinositol (PI), phosphatidylinositol-4-phosphate (PIP), and phosphatidylinositol-4,5-bisphosphate (PIP ). Growth factors and hormones trigger this phosphorylation event, which in turn coordinates cell growth, cell cycle entry, cell migration, and cell survival (1). PTEN reverses this process, and research studies have shown that the PI3K signaling pathway is constitutively activated in human cancers that have loss of function of PTEN (2). PI3Ks are composed of a catalytic subunit (p110) and a regulatory subunit. Various isoforms of the catalytic subunit (p110α, p110β, p110γ, and p110δ) have been isolated, and the regulatory subunits that associate with p110α, p110β, and p110δ are p85α and p85β (3). In contrast, p110γ associates with a p101 regulatory subunit that is unrelated to p85. Furthermore, p110γ is activated by βγ subunits of heterotrimeric G proteins (4).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nCLAPO; CLOVE; CWS5; MCAP; MCM; MCMTC; MGC142161; MGC142163; p110-alpha; p110alpha; phosphatidylinositol 3-kinase, catalytic, 110-KD, alpha; phosphatidylinositol 3-kinase, catalytic, alpha polypeptide; Phosphatidylinositol 4,5-bisphosphate 3-kinase 110 kDa catalytic subunit alpha; Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform; Phosphatidylinositol-4,5-bisphosphate 3-kinase 110 kDa catalytic subunit alpha; phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha; Phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha isoform; phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit, alpha isoform; Phosphoinositide-3-kinase catalytic alpha polypeptide; phosphoinositide-3-kinase, catalytic, alpha polypeptide; PI3-kinase p110 subunit alpha; PI3-kinase subunit alpha; PI3K; PI3K-alpha; PI3Kalpha; PIK3CA; PK3CA; PtdIns-3-kinase p110; PtdIns-3-kinase subunit alpha; PtdIns-3-kinase subunit p110-alpha; Serine\/threonine protein kinase PIK3CA\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R B\nSENSITIVITY: Endogenous\nMW (kDa): 110\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46799497887913,"sku":"4249T","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-4249t","provider":"Iright","version":"1.0","type":"link"}