{"product_id":"cst-42533t","title":"CST,  42533T, DR4 (D9S1R) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying Trail-R1\/DR4. Validated for WB,IP,IF,F. Available in 2 sizes. Highly specific and rigorously validated in-house, DR4 (D9S1R) Rabbit Monoclonal Antibody (CST #42533) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nImmunoprecipitation: 1:100\nImmunofluorescence (Immunocytochemistry): 1:800\nFlow Cytometry (Fixed\/Permeabilized): 1:50\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed\/Permeabilized)\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nDR4 (D9S1R) Rabbit Monoclonal Antibody recognizes endogenous levels of total DR4 protein.\nSpecies Reactivity: Human\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with recombinant protein specific to the carboxy terminal, cytoplasmic domain of human DR4 protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nThe tumor necrosis factor receptor family, which includes TNF-RI, Fas, DR3, DR4, DR5, and DR6, plays an important role in the regulation of apoptosis in various physiological systems (1,2). The receptors are activated by a family of cytokines that include TNF, FasL, and TNF-related apoptosis-inducing ligand (TRAIL). They are characterized by a highly conserved extracellular region containing cysteine-rich repeats and a conserved intracellular region of about 80 amino acids termed the death domain (DD). The DD is important for transducing the death signal by recruiting other DD containing adaptor proteins (FADD, TRADD, RIP) to the death-inducing signaling complex (DISC), resulting in activation of caspases. DR4 (TRAIL-RI, TNFRSF10A) and DR5 (TRAIL-R2, TNFRSF10B) are receptors for the cytokine TRAIL. Both receptors contain death domains that recruit DISC complexes triggering caspase activation and apoptosis (3-6). The ability of TRAIL to selectively kill malignant cells has led to clinical studies involving TRAIL and receptor agonists (7).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nAPO2; CD261; cytotoxic TRAIL receptor; Death receptor 4; DR4; MGC9365; TNF receptor superfamily member 10a; TNF-related apoptosis inducing ligand receptor 1; TNF-related apoptosis-inducing ligand receptor 1; TNFRSF10A; TR10A; TRAIL receptor 1; TRAIL-R1; TRAILR-1; TRAILR1; Tumor necrosis factor receptor superfamily member 10A; tumor necrosis factor receptor superfamily member 10a variant 2; tumor necrosis factor receptor superfamily, member 10a\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H\nSENSITIVITY: Endogenous\nMW (kDa): 35-55\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46799498215593,"sku":"42533T","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-42533t","provider":"Iright","version":"1.0","type":"link"}