{"product_id":"cst-4270s","title":"CST,  4270S, Non-phospho (Active) beta-Catenin (Ser33\/37\/Thr41) Antibody","description":"Polyclonal Antibody for studying Catenin-beta (Ser33\/Ser37\/Thr41) non-phosphate. Validated for Western Blotting. Highly specific and rigorously validated in-house, Non-phospho (Active) beta-Catenin (Ser33\/37\/Thr41) Antibody (CST #4270) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nNon-phospho (Active) beta-Catenin (Ser33\/37\/Thr41) Antibody recognizes endogenous β-Catenin when residues Ser33, Ser37, and Thr41 are not phosphorylated. This antibody does not detect β-Catenin if tri-phosphorylated at Ser33\/Ser37\/Thr41. This antibody may also detect β-Catenin protein when singly phosphorylated at Ser33. This specificity data was derived from competition ELISA and dot blot analysis using synthetic peptides.\nSpecies Reactivity: Human, Mouse, Rat, Monkey\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nPolyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser37 of human β-catenin. Antibodies are purified by protein A and peptide affinity chromatography.\n\u003cb\u003eBackground\u003c\/b\u003e\nβ-catenin is a key downstream effector in the Wnt signaling pathway (1). It is implicated in two major biological processes in vertebrates: early embryonic development (2) and tumorigenesis (3). CK1 phosphorylates β-catenin at Ser45. This phosphorylation event primes β-catenin for subsequent phosphorylation by GSK-3β (4-6). GSK-3β destabilizes β-catenin by phosphorylating it at Ser33, Ser37, and Thr41 (7). Mutations at these sites result in the stabilization of β-catenin protein levels and have been found in many tumor cell lines (8).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\narmadillo; Beta-catenin; catenin (cadherin-associated protein), beta 1, 88kDa; catenin beta 1; Catenin beta-1; CTNB1; CTNNB; CTNNB1; DKFZp686D02253; EVR7; FLJ25606; FLJ37923; MRD19; NEDSDV\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R Mk\nSENSITIVITY: Endogenous\nMW (kDa): 92\nSOURCE: Rabbit","brand":"CST","offers":[{"title":"Default Title","offer_id":46799499362473,"sku":"4270S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-4270s","provider":"Iright","version":"1.0","type":"link"}