{"product_id":"cst-4272s","title":"CST,  4272S, Cytochrome c Antibody","description":"Polyclonal Antibody for studying cytochrome C. Validated for Western Blotting,Immunohistochemistry (Paraffin). Available in 2 sizes. Highly specific and rigorously validated in-house, Cytochrome c Antibody (CST #4272) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nImmunohistochemistry (Paraffin): 1:200\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunohistochemistry (Paraffin)\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nCytochrome c Antibody detects endogenous levels of cytochrome c protein.\nSpecies Reactivity: Human, Mouse, Rat, Monkey, D. melanogaster\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nPolyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro72 of human cytochrome c. Antibodies are purified by protein A and peptide affinity chromatography.   Â\n\u003cb\u003eBackground\u003c\/b\u003e\nCytochrome c is a well conserved electron-transport protein and is part of the respiratory chain localized to mitochondrial intermembrane space (1). Upon apoptotic stimulation, cytochrome c released from mitochondria associates with procaspase-9 (47 kDa)\/Apaf 1. This complex processes caspase-9 from inactive proenzyme to its active form (2). This event further triggers caspase-3 activation and eventually leads to apoptosis (3).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nCYC; CYCS; Cytochrome c; cytochrome c, somatic; HCS; THC4\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R Mk Dm\nSENSITIVITY: Endogenous\nMW (kDa): 14\nSOURCE: Rabbit","brand":"CST","offers":[{"title":"Default Title","offer_id":46799499591849,"sku":"4272S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-4272s","provider":"Iright","version":"1.0","type":"link"}