{"product_id":"cst-42749s","title":"CST,  42749S, CDK4 (D9G3E) Rabbit Monoclonal Antibody (PE Conjugate)","description":"Monoclonal Antibody for studying Cdk4. Validated for Flow Cytometry (Fixed\/Permeabilized). Highly specific and rigorously validated in-house, CDK4 (D9G3E) Rabbit Monoclonal Antibody (PE Conjugate) (CST #42749) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nFlow Cytometry (Fixed\/Permeabilized): 1:50\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in PBS (pH 7.2), less than 0.1% sodium azide and 2 mg\/ml BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Flow Cytometry (Fixed\/Permeabilized)\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nCDK4 (D9G3E) Rabbit Monoclonal Antibody (PE Conjugate) recognizes endogenous levels of total CDK4 protein. In COS-7 cells, this antibody recognizes a 40 kDa background band of unknown origin. This antibody conjugate does not cross-react with CDK1, 2, or 6.\nSpecies Reactivity: Human, Monkey\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human CDK4 protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nCyclin-dependent kinase activity is regulated by T-loop phosphorylation (Thr172 in the case of CDK4), by the abundance of their cyclin partners, and by association with CDK inhibitors of the Cip\/Kip or INK family of proteins (1). The inactive ternary complex of CDK4\/cyclin D and p27 Kip1\/Cip1 requires extracellular mitogenic stimuli for the release and degradation of p27, which affects progression through the restriction point and pRb-dependent entry into S-phase (2). The active complex of CDK4\/cyclin D targets the retinoblastoma protein for phosphorylation, allowing the release of E2F transcription factors that activate G1\/S-phase gene expression (3). In HeLa cells, upon UV irradiation, upregulation of p16 INK4A association with CDK4\/cyclin D3 leads to a G2 delay, implicating CDK4\/cyclin D3 activity in progression through the G2-phase of the cell cycle (4).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nCDK4; cell division kinase 4; Cell division protein kinase 4; CMM3; cyclin dependent kinase 4; Cyclin-dependent kinase 4; melanoma cutaneous malignant, 3; MGC14458; PSK-J3\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H Mk\nSENSITIVITY: Endogenous\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46800400089257,"sku":"42749S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-42749s","provider":"Iright","version":"1.0","type":"link"}