{"product_id":"cst-43069s","title":"CST,  43069S, Intersectin-2 (E5J9F) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying ITSN2. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, Intersectin-2 (E5J9F) Rabbit Monoclonal Antibody (CST #43069) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nImmunoprecipitation: 1:100\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nIntersectin-2 (E5J9F) Rabbit Monoclonal Antibody recognizes endogenous levels of total intersectin-2 protein. This antibody reacts with intersectin-2L and intersectin-2S protein isoforms but does not cross-react with the corresponding isoforms of intersectin-1 protein. This antibody cross-reacts with proteins of unknown identity between 80 kDa and 120 kDa in some rat cell extracts.\nSpecies Reactivity: Human, Mouse, Rat\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Lys335 of human intersectin-2 protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nIntersectin-2 is a ubiquitously expressed, modular adaptor protein that participates in clathrin-mediated endocytosis through interaction with multiple proteins involved in trafficking of proteins through the endocytic pathway. Like its paralog, intersectin-1, there are two major isoforms of mammalian intersectin-2. Intersectin-2L and intersectin-2S are generated through alternative splicing of mRNA transcripts. The structure of both isoforms is characterized by two N-terminal EH domains, a central coiled-coil domain, followed by five tandem SH3 domains. The longer isoform, intersection-2L, contains an extended C-terminus with a Rho-GEF domain that controls the activity of the Cdc42 GTPase, a PH domain, and a calcium binding C2 domain (1-3). Research studies have shown that intersectin-2 plays a role in T cell receptor (TCR) signaling by promoting endocytosis of the TCR following its activation (4).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nintersectin 2; Intersectin-2; ITSN2; KIAA1256; PRO2015; SH3 domain protein 1B; SH3 domain-containing protein 1B; SH3D1B; SH3P18; SH3P18-like WASP associated protein; SH3P18-like WASP-associated protein; SWA; SWAP\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R\nSENSITIVITY: Endogenous\nMW (kDa): 200, 140\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46799501983913,"sku":"43069S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-43069s","provider":"Iright","version":"1.0","type":"link"}