{"product_id":"cst-43095s","title":"CST,  43095S, C\/EBP beta (E2K1U) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying C\/EBP-beta. Validated for WB,IHC,IF,F. Available in 2 sizes. Highly specific and rigorously validated in-house, C\/EBP beta (E2K1U) Rabbit Monoclonal Antibody (CST #43095) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nImmunohistochemistry (Paraffin): 1:400 - 1:1600\nImmunofluorescence (Immunocytochemistry): 1:400 - 1:1600\nFlow Cytometry (Fixed\/Permeabilized): 1:3200 - 1:6400\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 76191 .\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunohistochemistry (Paraffin), Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed\/Permeabilized)\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nC\/EBP beta (E2K1U) Rabbit Monoclonal Antibody recognizes endogenous levels of total C\/EBPβ protein. This antibody does not cross-react with C\/EBPα protein.\nSpecies Reactivity: Human, Monkey\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly67 of human C\/EBPβ protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nC\/EBPβ is a member of the transcription factor family of CCAAT\/enhancer-binding proteins (C\/EBPs) that are critical for cellular differentiation and function (1). There are various N-terminally truncated C\/EBPβ isoforms: full-length C\/EBPβ, C\/EBPβ-LAP (liver-enriched activator protein), and C\/EBPβ-LIP (liver-enriched inhibitory protein). In triple-negative breast cancer cells, aerobic glycolysis was shown to control the expression of C\/EBPβ-LAP, which in turn stimulates the expression of granulocyte colony-stimulating factor (G-CSF) and granulocyte macrophage colony-stimulating factor (GM-CSF). These factors are secreted from the cancer cells to promote myeloid-derived suppressor cell (MDSC) development in the tumor microenvironment, thereby suppressing anti-tumor immunity (2). In addition, research studies showed that decreased C\/EBPβ-LIP expression delays age-related phenotypes in mice, suggesting a potential role for C\/EBPβ-LIP in aging (3).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nC\/EBP beta; C\/EBP-beta; CCAAT enhancer binding protein beta; CCAAT\/enhancer binding protein (C\/EBP), beta; CCAAT\/enhancer-binding protein beta; CEBPB; CRP2; IL6DBP; interleukin 6-dependent DNA-binding protein; LAP; LIP; Liver activator protein; Liver-enriched inhibitory protein; liver-enriched transcriptional activator protein; MGC32080; NF-IL6; Nuclear factor NF-IL6; nuclear factor of interleukin 6; TCF-5; TCF5; Transcription factor 5; transcription factor C\/EBP beta\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H Mk\nSENSITIVITY: Endogenous\nMW (kDa): 46 (LAP), 48 (Full Length)\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46799502377129,"sku":"43095S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-43095s","provider":"Iright","version":"1.0","type":"link"}