{"product_id":"cst-43145s","title":"CST,  43145S, CD19 (1D3) Rat Monoclonal Antibody (PerCP-Cy5.5® Conjugate)","description":"Monoclonal Antibody for studying CD19 mouse. Validated for Flow Cytometry (Fixed\/Permeabilized),Flow Cytometry (Live). Highly specific and rigorously validated in-house, CD19 (1D3) Rat Monoclonal Antibody (PerCP-Cy5.5\u003csup\u003e®\u003c\/sup\u003e Conjugate) (CST #43145) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nFor optimal flow cytometry results, we recommend 0.125 μg of antibody per test.\nFlow Cytometry (Fixed\/Permeabilized): 1:160\nFlow Cytometry (Live): 1:160\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM NaH 2 PO 4 , 150 mM NaCl, 0.09% NaN 3 , 0.1% gelatin, pH 7.2. This product is stable for 6 months when stored at 4ºC. Do not aliquot the antibody. Protect from light. Do not freeze.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Flow Cytometry (Fixed\/Permeabilized), Flow Cytometry (Live)\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nCD19 (1D3) Rat Monoclonal Antibody (PerCP-Cy5.5\nSpecies Reactivity: Mouse\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nThis monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.\n\u003cb\u003eBackground\u003c\/b\u003e\nCD19 is a 95 kDa coreceptor which amplifies the signaling cascade in B cells (1). On the B cell surface, CD19 associates with CD21, CD81, and Leu-13 to exert its function. The cytoplasmic tail of CD19 has nine conserved tyrosine residues playing critical roles in CD19 mediated function by coupling signaling molecules to the receptor (1). After B cell receptor or CD19 ligation, Tyr531 and Tyr500 of CD19 are progressively phosphorylated. This phosphorylation enables the coupling of PI3 kinase and Src family tyrosine kinase to CD19 and activates the PI3K and Src signaling pathways (2,3). Coligation of B cell receptor and CD19 also promotes Tyr409 phosphorylation in CD19. The phosphorylation at these sites enables its binding to Vav and mediates elevated intracellular calcium response, as well as the JNK pathway (4,5). The 1D3 clone is a widely used as a phenotypic marker for CD19 expression on the surface of murine B cells and subsets of dendritic cells.\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nAW495831; B-lymphocyte antigen CD19; Cd19; CD19 antigen; Differentiation antigen CD19\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: M\nSENSITIVITY: Endogenous\nSource\/Isotype: Rat IgG2a kappa","brand":"CST","offers":[{"title":"Default Title","offer_id":46800401039529,"sku":"43145S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-43145s","provider":"Iright","version":"1.0","type":"link"}