{"product_id":"cst-43212s","title":"CST,  43212S, IL2-R alpha\/CD25 (BC96) Mouse Monoclonal Antibody (PE-Cy7® Conjugate)","description":"Monoclonal Antibody for studying IL2RA. Validated for Flow Cytometry (Live). Highly specific and rigorously validated in-house, IL2-R alpha\/CD25 (BC96) Mouse Monoclonal Antibody (PE-Cy7\u003csup\u003e®\u003c\/sup\u003e Conjugate) (CST #43212) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nFlow Cytometry (Live): 1:20\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM NaH 2 PO 4 , 150 mM NaCl, 0.09% NaN 3 , 0.1% gelatin, pH 7.2. This product is stable for 6 months when stored at 4ºC. Do not aliquot the antibody. Protect from light. Do not freeze.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Flow Cytometry (Live)\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nIL2-R alpha\/CD25 (BC96) Mouse Monoclonal Antibody (PE-Cy7\nSpecies Reactivity: Human\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nThis monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.\n\u003cb\u003eBackground\u003c\/b\u003e\nInterleukin-2 (IL-2) is a T cell stimulatory cytokine best known for inducing T cell proliferation and NK cell proliferation and activation (1,2). IL-2 also promotes peripheral development of regulatory T cells (Tregs) (3,4). Conversely, IL-2 is involved in the activation-induced cell death (AICD) that is observed post T cell expansion by increasing levels of Fas on CD4 T cells (5). The effects of IL-2 are mediated through a trimeric receptor complex consisting of IL-2Rα, IL-2Rβ, and the common gamma chain, γc (1,2). IL-2Rα binds exclusively to IL-2 with low affinity and increases the binding affinity of the whole receptor complex including IL-2Rβ and γc subunits. IL-15 also binds to IL-2Rβ (1,2). γc is used by other cytokines, including IL-4, IL-7, IL-9, IL-15, and IL-21 (1,2). Binding of IL-2 initiates signaling cascades involving Jak1, Jak3, Stat5, and the PI3K\/Akt pathways (1,2).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nCD25; IDDM10; IL-2 receptor subunit alpha; IL-2-RA; IL-2R subunit alpha; IL2-RA; IL2R; IL2RA; IMD41; interleukin 2 receptor subunit alpha; interleukin 2 receptor, alpha; Interleukin-2 receptor subunit alpha; p55; TAC antigen; TCGFR\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H\nSENSITIVITY: Endogenous\nSource\/Isotype: Mouse IgG1 kappa","brand":"CST","offers":[{"title":"Default Title","offer_id":46800401301673,"sku":"43212S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-43212s","provider":"Iright","version":"1.0","type":"link"}