{"product_id":"cst-4327s","title":"CST,  4327S, Phospho-AML1 (Ser249) Antibody","description":"Polyclonal Antibody for studying AML1 (Ser249) phosphate. Validated for Western Blotting,Immunoprecipitation. Highly specific and rigorously validated in-house, Phospho-AML1 (Ser249) Antibody (CST #4327) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nImmunoprecipitation: 1:50\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nPhospho-AML1 (Ser249) Antibody detects endogenous levels of AML1 protein only when phosphorylated on Ser249.\nSpecies Reactivity: Human\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nPolyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to amino acids around Ser249 of human AML1. Antibodies are purified by protein A and peptide affinity chromatography.\n\u003cb\u003eBackground\u003c\/b\u003e\nAML1 (also known as Runx1, CBFA2, and PEBP2αB) is a member of the core binding factor (CBF) family of transcription factors (1,2). It is required for normal development of all hematopoietic lineages (3-5). AML1 forms a heterodimeric DNA binding complex with its partner protein CBFβ and regulates the expression of cellular genes by binding to promoter and enhancer elements. AML1 is commonly translocated in hematopoietic cancers: chromosomal translocations include t(8;21) AML1-ETO, t(12;21) TEL-AML, and t(8;21) AML-M2 (6). Phosphorylation of AML1 on several potential serine and threonine sites, including Ser249, is thought to occur in an Erk-dependent manner (7,8).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nAcute myeloid leukemia 1 protein; AML1; aml1 oncogene; AML1-EVI-1; AML1-EVI-1 fusion partner; AML1-EVI-1 fusion protein; AMLCR1; CBF-alpha-2; CBF2alpha; CBFA2; Core-binding factor subunit alpha-2; core-binding factor, runt domain, alpha subunit 2; EVI-1; mutant RUNX1; Oncogene AML-1; PEA2-alpha B; PEBP2-alpha B; PEBP2aB; PEBP2alpha; Polyomavirus enhancer-binding protein 2 alpha B subunit; runt related transcription factor 1; Runt-related transcription factor 1; RUNX family transcription factor 1; RUNX1; SL3-3 enhancer factor 1 alpha B subunit; SL3\/AKV core-binding factor alpha B subunit\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H\nSENSITIVITY: Endogenous\nMW (kDa): 55\nSOURCE: Rabbit","brand":"CST","offers":[{"title":"Default Title","offer_id":46799503753385,"sku":"4327S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-4327s","provider":"Iright","version":"1.0","type":"link"}