{"product_id":"cst-43306t","title":"CST,  43306T, COL2A1 (E8S2S) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying COL2A1. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, COL2A1 (E8S2S) Rabbit Monoclonal Antibody (CST #43306) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nCOL2A1 (E8S2S) Rabbit Monoclonal Antibody recognizes endogenous levels of total COL2A1 protein. This antibody detects a non-specific 62 kDa band with unknown origin.\nSpecies Reactivity: Human\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala194 of human COL2A1 protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nCOL2A1 (α1 type II collagen) is a fibril-forming collagen encoded by the gene (1,2). The protein forms homotrimeric type II collagen and is the main extracellular component in hyaline cartilage extracellular matrix (ECM), nucleus pulposus of intervertebral discus, vitreous humor of the eye, and inner ear structure (3). Type II collagen plays a fundamental role in maintaining cartilage formation, differentiation, and growth (4-6). Mutations of cause impairment of ECM deposition and signaling, which contribute to various forms of tissue dysplasia and pathologies such as Stickler syndrome and chondrosarcoma (3,7,8). At the ECM, COL2A1 interacts with integrins such as integrin β1 and growth factors such as IL1β, IL6, IL8, and others, which activate intracellular signaling pathways, including SMAD1, and MAP kinases p38, JNK, and ERK (9-12).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nAlpha-1 type II collagen; ANFH; AOM; arthroophthalmopathy, progressive (Stickler syndrome); cartilage collagen; Chondrocalcin; CO2A1; COL11A3; COL2A1; Collagen alpha-1(II) chain; collagen II; collagen II, alpha-1 polypeptide; collagen type II alpha 1 chain; collagen, type II, alpha 1; MGC131516; SEDC; STL1\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H\nSENSITIVITY: Endogenous\nMW (kDa): 200\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46799503982761,"sku":"43306T","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-43306t","provider":"Iright","version":"1.0","type":"link"}