{"product_id":"cst-4342s","title":"CST,  4342S, Pbx1 Antibody","description":"Polyclonal Antibody for studying PBX1. Validated for Western Blotting,Immunoprecipitation,Immunofluorescence (Immunocytochemistry). Highly specific and rigorously validated in-house, Pbx1 Antibody (CST #4342) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nImmunoprecipitation: 1:100\nImmunofluorescence (Immunocytochemistry): 1:400\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Immunocytochemistry)\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nPbx1 Antibody detects endogenous levels of total Pbx1a and Pbx1b proteins.\nSpecies Reactivity: Human, Mouse\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nPolyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly29 of human Pbx1 protein. Antibodies are purified by protein A and peptide affinity chromatography\n\u003cb\u003eBackground\u003c\/b\u003e\nThe Pbxs (Pre-B cell leukemia transcription factors) belong to the Three Amino Acid Loop Extension (TALE) family of homeodomain containing protein (1). Pbx1 has two isoforms, Pbx1a and 1b, that are divergent in sequence at the carboxy terminus, which is a result of alternative mRNA splicing. Unlike other homeoselector genes that have restricted expression in development Pbx genes are ubiquitously expressed in both fetal and adult tissue (1). Human pre-B cell acute leukemias are frequently associated with a t(1;19)(q23;p13.3) translocation that results in a fusion between the E2A and Pbx1 genes (2,3). Pbx1 genes are not normally expressed in lymphoid tissues, and the E2A-Pbx1 fusion protein is a potent transcription factor that drives aberrant gene expression.\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nCAKUHED; DKFZp686B09108; Homeobox protein PBX1; Homeobox protein PRL; MGC126627; PBX homeobox 1; PBX1; Pre-B cell leukemia transcription factor-1; pre-B-cell leukemia homeobox 1; Pre-B-cell leukemia transcription factor 1; PRL\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M\nSENSITIVITY: Endogenous\nMW (kDa): 42, 52\nSOURCE: Rabbit","brand":"CST","offers":[{"title":"Default Title","offer_id":46799504998569,"sku":"4342S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-4342s","provider":"Iright","version":"1.0","type":"link"}