{"product_id":"cst-43640s","title":"CST,  43640S, DNA Ligase III (F2A1H) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying LIG3. Validated for Western Blotting,Simple Western™,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, DNA Ligase III (F2A1H) Rabbit Monoclonal Antibody (CST #43640) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nSimple Western™: 1:10 - 1:50\nImmunoprecipitation: 1:50\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nDNA Ligase III (F2A1H) Rabbit Monoclonal Antibody recognizes endogenous levels of total DNA ligase III protein.\nSpecies Reactivity: Human, Mouse, Rat\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human DNA ligase III protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nDNA ligase III\/LIG3 is an ATP-dependent DNA ligase that functions in DNA repair. Four isoforms of LIG3 result from alternative splicing\/initiation, including a mitochondrial-specific isoform essential for cell viability (1). Loss of nuclear LIG3 sensitizes resistant cells to PARP inhibitors and enhances PARP inhibitor efficacy in BRCA1-deficient mouse mammary tumors (2). In multiple myeloma (MM), LIG3 is crucial for repair of DNA double-stranded breaks (DSBs) via alternative-non-homologous end joining (Alt-NHEJ), and LIG3 inhibitors are being investigated as cancer therapeutics (3).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nDNA ligase 3; DNA ligase III; DNLI3; LIG2; LIG3; LIG3alpha; ligase II, DNA, ATP-dependent; ligase III, DNA, ATP-dependent; Polydeoxyribonucleotide synthase [ATP] 3\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R\nSENSITIVITY: Endogenous\nMW (kDa): 100\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46799506604201,"sku":"43640S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-43640s","provider":"Iright","version":"1.0","type":"link"}