{"product_id":"cst-43720w2","title":"CST,  43720W2, Tri-Methyl-Histone H3 (Lys4) (C42D8) Rabbit Monoclonal Antibody (SignalFlex™ mFluor™ Violet 510 Conjugate)","description":"Monoclonal Antibody for studying H3 (Lys4) trimethylate. Highly specific and rigorously validated in-house, Tri-Methyl-Histone H3 (Lys4) (C42D8) Rabbit Monoclonal Antibody (SignalFlex\u003csup\u003e™\u003c\/sup\u003e mFluor\u003csup\u003e™\u003c\/sup\u003e Violet 510 Conjugate) (CST #43720) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nSignalFlex™ conjugates are produced using highly validated Cell Signaling Technology ® primary antibodies and conjugation methods that have been rigorously tested, ensuring high-quality conjugates and lot-to-lot consistency. These conjugates are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. However, they are not tested on specific assays. Optimal dilutions\/concentrations should be determined by the end user. When performing flow cytometry, we recommend using an isotype control conjugate at the same concentration as the antibody conjugate.\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in PBS (pH 7.2), less than 0.1% sodium azide, and 2 mg\/mL BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nTri-Methyl-Histone H3 (Lys4) (C42D8) Rabbit Monoclonal Antibody (SignalFlex™ mFluor™ Violet 510 Conjugate) detects endogenous levels of histone H3 when tri-methylated on Lys4. This antibody shows some cross-reactivity with histone H3 that is di-methylated on Lys4, but does not cross-react with non-methylated or mono-methylated histone H3 Lys4. In addition, the antibody does not cross-react with methylated histone H3 Lys9, Lys27, Lys36 or methylated histone H4 Lys20.\nSpecies Reactivity: Human, Mouse, Rat, Monkey, D. melanogaster, S. cerevisiae\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the amino terminus of histone H3 in which Lys4 is tri-methylated.\n\u003cb\u003eBackground\u003c\/b\u003e\nThe nucleosome, made up of four core histone proteins (H2A, H2B, H3, and H4), is the primary building block of chromatin. Originally thought to function as a static scaffold for DNA packaging, histones have now been shown to be dynamic proteins, undergoing multiple types of post-translational modifications, including acetylation, phosphorylation, methylation, and ubiquitination (1). Histone methylation is a major determinant for the formation of active and inactive regions of the genome and is crucial for the proper programming of the genome during development (2,3). Arginine methylation of histones H3 (Arg2, 17, 26) and H4 (Arg3) promotes transcriptional activation and is mediated by a family of protein arginine methyltransferases (PRMTs), including the co-activators PRMT1 and CARM1 (PRMT4) (4). In contrast, a more diverse set of histone lysine methyltransferases has been identified, all but one of which contain a conserved catalytic SET domain originally identified in the Su(var)3-9, Enhancer of zeste, and Trithorax proteins. Lysine methylation occurs primarily on histones H3 (Lys4, 9, 27, 36, 79) and H4 (Lys20) and has been implicated in both transcriptional activation and silencing (4). Methylation of these lysine residues coordinates the recruitment of chromatin modifying enzymes containing methyl-lysine binding modules such as chromodomains (HP1, PRC1), PHD fingers (BPTF, ING2), tudor domains (53BP1), and WD-40 domains (WDR5) (5-8). The discovery of histone demethylases, such as PADI4, LSD1, JMJD1, JMJD2, and JHDM1, has shown that methylation is a reversible epigenetic marker (9).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nH3; H3 clustered histone 1; H3 histone family, member A; H3\/A; H31; H3C1; H3C10; H3C11; H3C12; H3C2; H3C3; H3C4; H3C6; H3C7; H3C8; H3FA; H3FB; H3FC; H3FC HIST1H3C; H3FD; H3FF; H3FH; H3FI; H3FJ; H3FK; H3FL; HIST1H3A; HIST1H3B; HIST1H3C; HIST1H3D; HIST1H3E; HIST1H3F; HIST1H3G; HIST1H3H; HIST1H3I; HIST1H3J; histone 1, H3a; histone cluster 1 H3 family member a; histone cluster 1, H3a; Histone H3; Histone H3.1; Histone H3\/a; Histone H3\/b; Histone H3\/c; Histone H3\/d; Histone H3\/f; Histone H3\/h; Histone H3\/i; Histone H3\/j; Histone H3\/k; Histone H3\/l\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R Mk Dm Sc\nSENSITIVITY: Endogenous\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46800402514089,"sku":"43720W2","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-43720w2","provider":"Iright","version":"1.0","type":"link"}