{"product_id":"cst-4372s","title":"CST,  4372S, p44 MAP Kinase (Erk1) Antibody","description":"Polyclonal Antibody for studying ERK1. Validated for Western Blotting,Immunoprecipitation. Highly specific and rigorously validated in-house, p44 MAP Kinase (Erk1) Antibody (CST #4372) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nImmunoprecipitation: 1:50\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\np44 MAP Kinase (Erk1) Antibody detects endogenous levels of p44 MAP kinase (Erk1). This antibody does not recognize p38 MAP kinase, p42 MAP kinase (Erk2) or SAPK\/JNK.\nSpecies Reactivity: Mouse, Rat\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nPolyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the N-terminus of p44 MAP kinase (Erk1). Antibodies are purified by protein A and peptide affinity chromatography.\n\u003cb\u003eBackground\u003c\/b\u003e\nMitogen-activated protein kinases (MAPKs) are a widely conserved family of serine\/threonine protein kinases involved in many cellular programs, such as cell proliferation, differentiation, motility, and death. The p44\/42 MAPK (Erk1\/2) signaling pathway can be activated in response to a diverse range of extracellular stimuli, including mitogens, growth factors, and cytokines (1-3), and research investigators consider it an important target in the diagnosis and treatment of cancer (4). Upon stimulation, a sequential three-part protein kinase cascade is initiated, consisting of a MAP kinase kinase kinase (MAPKKK or MAP3K), a MAP kinase kinase (MAPKK or MAP2K), and a MAP kinase (MAPK). Multiple p44\/42 MAP3Ks have been identified, including members of the Raf family, as well as Mos and Tpl2\/COT. MEK1 and MEK2 are the primary MAPKKs in this pathway (5,6). MEK1 and MEK2 activate p44 and p42 through phosphorylation of activation loop residues Thr202\/Tyr204 and Thr185\/Tyr187, respectively. Several downstream targets of p44\/42 have been identified, including p90RSK (7) and the transcription factor Elk-1 (8,9). p44\/42 are negatively regulated by a family of dual-specificity (Thr\/Tyr) MAPK phosphatases, known as DUSPs or MKPs (10), along with MEK inhibitors, such as U0126 and PD98059.\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nERK; ERK-1; ERK1; ERT2; Extracellular signal-regulated kinase 1; extracellular signal-related kinase 1; HS44KDAP; HUMKER1A; Insulin-stimulated MAP2 kinase; MAP kinase 1; MAP kinase 3; MAP kinase isoform p44; MAPK 1; MAPK 3; MAPK3; MGC20180; Microtubule-associated protein 2 kinase; Mitogen-activated protein kinase 1; Mitogen-activated protein kinase 3; MK03; p44-ERK1; p44-MAPK; P44ERK1; P44MAPK; PRKM3\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: M R\nSENSITIVITY: Endogenous\nMW (kDa): 44\nSOURCE: Rabbit","brand":"CST","offers":[{"title":"Default Title","offer_id":46800096657577,"sku":"4372S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-4372s","provider":"Iright","version":"1.0","type":"link"}