{"product_id":"cst-4374s","title":"CST,  4374S, Phospho-p44\/42 MAPK (Erk1\/2) (Thr202\/Tyr204) (E10) Mouse Monoclonal Antibody (Alexa Fluor® 488 Conjugate)","description":"Monoclonal Antibody for studying ERK1 (Thr202\/Tyr204) phosphate\/ERK2 (Thr185\/Tyr187) phosphate. Validated for Flow Cytometry (Fixed\/Permeabilized). Highly specific and rigorously validated in-house, Phospho-p44\/42 MAPK (Erk1\/2) (Thr202\/Tyr204) (E10) Mouse Monoclonal Antibody (Alexa Fluor\u003csup\u003e®\u003c\/sup\u003e 488 Conjugate) (CST #4374) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nFlow Cytometry (Fixed\/Permeabilized): 1:50\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in PBS (pH 7.2), less than 0.1% sodium azide and 2 mg\/ml BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Flow Cytometry (Fixed\/Permeabilized)\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nPhospho-p44\/42 MAPK (Erk1\/2) (Thr202\/Tyr204) (E10) Mouse Monoclonal Antibody (Alexa Fluor\nSpecies Reactivity: Human, Mouse, Rat, Hamster, Monkey, Mink, Zebrafish, Bovine, Pig\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr202\/Tyr204 of human p44 MAP kinase. The antibody was conjugated to Alexa Fluor 488 under optimal conditions with an F\/P ratio of 2-6.\n\u003cb\u003eBackground\u003c\/b\u003e\nMitogen-activated protein kinases (MAPKs) are a widely conserved family of serine\/threonine protein kinases involved in many cellular programs, such as cell proliferation, differentiation, motility, and death. The p44\/42 MAPK (Erk1\/2) signaling pathway can be activated in response to a diverse range of extracellular stimuli, including mitogens, growth factors, and cytokines (1-3), and research investigators consider it an important target in the diagnosis and treatment of cancer (4). Upon stimulation, a sequential three-part protein kinase cascade is initiated, consisting of a MAP kinase kinase kinase (MAPKKK or MAP3K), a MAP kinase kinase (MAPKK or MAP2K), and a MAP kinase (MAPK). Multiple p44\/42 MAP3Ks have been identified, including members of the Raf family, as well as Mos and Tpl2\/COT. MEK1 and MEK2 are the primary MAPKKs in this pathway (5,6). MEK1 and MEK2 activate p44 and p42 through phosphorylation of activation loop residues Thr202\/Tyr204 and Thr185\/Tyr187, respectively. Several downstream targets of p44\/42 have been identified, including p90RSK (7) and the transcription factor Elk-1 (8,9). p44\/42 are negatively regulated by a family of dual-specificity (Thr\/Tyr) MAPK phosphatases, known as DUSPs or MKPs (10), along with MEK inhibitors, such as U0126 and PD98059.\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nERK; ERK-1; ERK-2; ERK1; ERK2; ERT1; ERT2; Extracellular signal-regulated kinase 1; Extracellular signal-regulated kinase 2; extracellular signal-regulated kinase-2; extracellular signal-related kinase 1; HS44KDAP; HUMKER1A; Insulin-stimulated MAP2 kinase; MAP kinase 1; MAP kinase 2; MAP kinase 3; MAP kinase isoform p42; MAP kinase isoform p44; MAPK 1; MAPK 2; MAPK 3; MAPK1; MAPK2; MAPK3; MGC20180; Microtubule-associated protein 2 kinase; Mitogen-activated protein kinase 1; Mitogen-activated protein kinase 2; Mitogen-activated protein kinase 3; MK01; MK03; p38; p40; p41; p41mapk; p42-MAPK; P42MAPK; p44-ERK1; p44-MAPK; P44ERK1; P44MAPK; PRKM1; PRKM2; PRKM3; protein tyrosine kinase ERK2\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R Hm Mk Mi Z B Pg\nSENSITIVITY: Endogenous\nSource\/Isotype: Mouse IgG1","brand":"CST","offers":[{"title":"Default Title","offer_id":46800402612393,"sku":"4374S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-4374s","provider":"Iright","version":"1.0","type":"link"}