{"product_id":"cst-43816sf","title":"CST,  43816SF, MHC Class II (LGII-612.14) Mouse Monoclonal Antibody (BSA and Azide Free)","description":"Monoclonal Antibody for studying HLA-DRB1\/HLA-DPB1. Validated for WB,IHC,IHC,F. Highly specific and rigorously validated in-house, MHC Class II (LGII-612.14) Mouse Monoclonal Antibody (BSA and Azide Free) (CST #43816) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nThis product is the carrier free version of product #68258. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT\u0026amp;RUN, or CUT\u0026amp;Tag assays. If you require a carrier-free formulation for chromatin profiling, please contact us . Optimal dilutions\/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity.\n\u003cb\u003eFormulation\u003c\/b\u003e\nSupplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 68258 .\n\u003cb\u003eStorage\u003c\/b\u003e\nStore at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze\/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance.\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nMHC Class II (LGII-612.14) Mouse Monoclonal Antibody (BSA and Azide Free) exhibits strong reactivity with HLA-DRB and weak reactivity with HLA-DPB in cell lines transfected with constructs expressing Myc\/DDK-tagged HLA-DRB and HLA-DPB, respectively. Reactivity is not observed with HLA-DMB, HLA-DOB, and HLA-DQB in cell lines transfected with constructs expressing Myc\/DDK-tagged HLA-DMB, HLA-DOB, and HLA-DQB.\nSpecies Reactivity: Human\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with cultured human B lymphoid cells treated with IFN-gamma.\n\u003cb\u003eBackground\u003c\/b\u003e\nMajor histocompatibility complex class II (MHC class II) molecules are heterodimeric, transmembrane glycoproteins expressed on the surface of antigen-presenting cells such as macrophages, dendritic cells, and B cells. Expression can also be induced on other cell types through interferon-γ signaling (1). Prior to being displayed on the cell membrane, MHC class II molecules are loaded with exogenous peptide antigens approximately 15-24 amino acids in length that were derived from endocytosed extracellular proteins digested in the lysosome (2). Antigen-presentation through MHC class II is required for T cell activation during the immune response to extracellular pathogens (2). In humans, the MHC class II protein complex is encoded by the human leukocyte antigen gene complex (HLA). HLAs corresponding to MHC class II are HLA-DP, HLA-DM, HLA-DOA, HLA-DOB, HLA-DQ, and HLA-DR (3). In the literature, this clone is reported to react with HLA-DR, HLA-DP, and HLA-DQ (4).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\n2B11; cell surface glycoprotein; clone P2-beta-3; Clone P2-beta-4; DPB1; DR-1; DR-16; DR-5; DR-8; DR1; DR16; DR5; DR8; DRB; DRB1; DRw10; DRw11; DRw8; FLJ75017; FLJ76359; HLA class II antigen beta chain; HLA class II histocompatibility antigen, DP beta 1 chain; HLA class II histocompatibility antigen, DP(W4) beta chain; HLA class II histocompatibility antigen, DR-1 beta chain; HLA class II histocompatibility antigen, DRB1 beta chain; HLA class II histocompatibility antigen, DRB1-1 beta chain; HLA class II histocompatibility antigen, DRB1-10 beta chain; HLA class II histocompatibility antigen, DRB1-11 beta chain; HLA class II histocompatibility antigen, DRB1-16 beta chain; HLA class II histocompatibility antigen, DRB1-3 chain; HLA class II histocompatibility antigen, DRB1-8 beta chain; HLA-DP; HLA-DP histocompatibility type, beta-1 subunit; HLA-DP1B; HLA-DPB; HLA-DPB1; HLA-DR-beta 1; HLA-DR1B; HLA-DRB; HLA-DRB1; human leucocyte antigen DRB1; Human leukocyte antigen DRB1; leucocyte antigen DR beta 1 chain; leucocyte antigen DRB1; lymphocyte antigen DRB1; major histocompatibility complex, class II, DP beta 1; major histocompatibility complex, class II, DR beta 1; major histocompatibility complex, class II, DR beta 1 precursor; MHC class I antigen DRB1; MHC class II antigen DPB1; MHC class II antigen DRB1; MHC class II antigen HLA-DR13; MHC class II HLA-DP-beta-1; MHC class II HLA-DR beta 1 chain; MHC class II HLA-DR-beta cell surface glycoprotein; MHC class II HLA-DRw10-beta; MHC HLA DPB1; SS1\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H\nSENSITIVITY: Endogenous\nMW (kDa): 25-35, 50-65\nSource\/Isotype: Mouse IgG1","brand":"CST","offers":[{"title":"Default Title","offer_id":46799507816617,"sku":"43816SF","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-43816sf","provider":"Iright","version":"1.0","type":"link"}