{"product_id":"cst-43996s","title":"CST,  43996S, Furin Antibody","description":"Polyclonal Antibody for studying FURIN. Validated for Western Blotting,Immunoprecipitation. Highly specific and rigorously validated in-house, Furin Antibody (CST #43996) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nImmunoprecipitation: 1:50\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nFurin Antibody recognizes endogenous levels of total furin protein.\nSpecies Reactivity: Human, Mouse, Rat\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nPolyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala237 of human furin protein. Antibodies are purified by peptide affinity chromatography.\n\u003cb\u003eBackground\u003c\/b\u003e\nThe proprotein convertases (PCs) are enzymes that activate precursor proteins through proteolytic cleavage within the secretory pathway. PCs comprise several enzymes that are basic amino acid-specific proteinases (furin, PC1\/3, PC2, PC4, PACE4, PC5\/6, and PC7), as well as nonbasic amino acid convertases (S1P and PC9) (1). PCs have a common structure that includes an N-terminal signal peptide for secretory pathway targeting; a pro-domain that is thought to act as an intramolecular chaperone; a catalytic domain containing the active site; a P-domain that contributes to the overall folding of the enzyme by regulating stability and both calcium- and pH-dependence; and a C-terminal domain that interacts with the membrane (2). PCs act in a tissue- and substrate-specific fashion to generate an array of bioactive peptides and proteins from precursors, both in the brain and in peripheral tissues (3). The SARS-CoV-2 coronavirus contains an inactive precursor spike glycoprotein, with a distinct furin cleavage site at the S1\/S2 domain junction. Cleavage by furin \"primes\" the spike protein for binding to the ACE2 receptor and subsequent viral entry to the host cell (4-6). Loss of the furin cleavage site has been shown to drastically reduce the virulence of the SARS-CoV-2 virus (6-8). Furin cleavage sites are seen in a variety of other viral pathogens as well, including other CoV family members, HIV, avian influenza strains, and Ebola (6,8). In addition, furin has been proposed as a therapeutic target in cancer, and in regard to NMDA receptor-associated pathologies in the brain (9,10).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\ndibasic processing enzyme; Dibasic-processing enzyme; FES upstream region; FUR; Furin; furin (paired basic amino acid cleaving enzyme); furin, membrane associated receptor protein; furin, paired basic amino acid cleaving enzyme; PACE; Paired basic amino acid residue-cleaving enzyme; PCSK3; proprotein convertase subtilisin\/kexin type 3; SPC1\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R\nSENSITIVITY: Endogenous\nMW (kDa): 90\nSOURCE: Rabbit","brand":"CST","offers":[{"title":"Default Title","offer_id":46799509356713,"sku":"43996S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-43996s","provider":"Iright","version":"1.0","type":"link"}