{"product_id":"cst-4441t","title":"CST,  4441T, Phospho-Stat2 (Tyr690) Antibody","description":"Polyclonal Antibody for studying Stat2 (Tyr690) phosphate. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, Phospho-Stat2 (Tyr690) Antibody (CST #4441) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nPhospho-Stat2 (Tyr690) Antibody detects endogenous levels of human Stat2 only when phosphorylated at Tyr690. This antibody does not significantly cross-react with corresponding phospho-tyrosine sites on other Stat proteins.\nSpecies Reactivity: Human\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nPolyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr690 of human Stat2 protein. Antibodies are purified by protein A and peptide affinity chromatography.\n\u003cb\u003eBackground\u003c\/b\u003e\nStat2 (113 kDa), originally purified from the nuclei of alpha-interferon-treated cells, is critical to the transcriptional responses induced by type I interferons, IFN-alpha\/beta (1,2). Knockout mice with a targeted disruption of Stat2 have higher susceptibility to viral infection and altered responses to type I interferons (3). Stat2 is rapidly activated by phosphorylation at Tyr690 in response to stimulation by IFN-alpha\/beta via associations with receptor-bound Jak kinases (4). Unlike other Stat proteins, Stat2 does not form homodimers. Instead, activated Stat2 forms a heterodimer with Stat1 and translocates to the nucleus. There, it associates with the DNA-binding protein p48 and forms the transcriptional activator complex, interferon-stimulated gene factor 3 (ISGF3), promoting transcription from the ISRE (5).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nIMD44; interferon alpha induced transcriptional activator; ISGF-3; MGC59816; p113; PTORCH3; Signal transducer and activator of transcription 2; signal transducer and activator of transcription 2, 113kDa; STAT113; STAT2\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H\nSENSITIVITY: Endogenous\nMW (kDa): 113\nSOURCE: Rabbit","brand":"CST","offers":[{"title":"Default Title","offer_id":46799512010921,"sku":"4441T","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-4441t","provider":"Iright","version":"1.0","type":"link"}