{"product_id":"cst-44627s","title":"CST,  44627S, Chitotriosidase-1\/CHIT1 (E4X4I) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying Chitinase-1. Validated for Western Blotting,Simple Western™. Available in 2 sizes. Highly specific and rigorously validated in-house, Chitotriosidase-1\/CHIT1 (E4X4I) Rabbit Monoclonal Antibody (CST #44627) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nSimple Western™: 1:10 - 1:50\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nChitotriosidase-1\/CHIT1 (E4X4I) Rabbit Monoclonal Antibody recognizes endogenous levels of total Chitotriosidase-1\/CHIT1 protein.\nSpecies Reactivity: Human\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser406 of human Chitotriosidase-1\/CHIT1 protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nChitin is an essential biopolymer structural component of many organisms, including arthropods, fungi, and parasites. Chitinases are part of the 18-glycoside hydrolase (18-GH) family of proteins that act to bind and degrade chitin. In mammals, this function provides defense against chitin-containing pathogens. Chitotriosidase-1, also known as Chitinase-1 or CHIT1, is the most abundant of these enzymes. CHIT1 consists of an N-terminal catalytic domain, a C-terminal chitin-binding domain, and a short hinge region that connects the two. CHIT1 activity is implicated in several inflammatory signaling pathways and is considered a sensitive biomarker for activated immune cells. It is produced and secreted by macrophages, microglia, and neutrophils in normal and inflammatory conditions. Elevated levels of CHIT1 in serum is a suggested biomarker for Gaucher's disease, atherosclerosis, pulmonary fibrosis, and some cancers. Elevated CSF levels of CHIT1 is also a suggested biomarker for amyotrophic lateral sclerosis (ALS) and other neurodegenerative diseases (1-5).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nCHI3; CHIT; CHIT1; CHITD; chitinase 1; chitinase 1 (chitotriosidase); Chitinase-1; Chitotriosidase-1; FLJ00314; MGC125322; plasma methylumbelliferyl tetra-N-acetylchitotetraoside hydrolase\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H\nSENSITIVITY: Endogenous\nMW (kDa): 50\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46799513944233,"sku":"44627S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-44627s","provider":"Iright","version":"1.0","type":"link"}