{"product_id":"cst-4470s","title":"CST,  4470S, RagD Antibody","description":"Polyclonal Antibody for studying RagD. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, RagD Antibody (CST #4470) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nRagD Antibody detects endogenous levels of total RagD protein.\nSpecies Reactivity: Human\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nPolyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser37 of human RagD protein. Antibodies are purified by protein A and peptide affinity chromatography.\n\u003cb\u003eBackground\u003c\/b\u003e\nThe mTORC1 kinase complex is a critical regulator of cell growth (1,2). Its activity is modulated by energy levels, growth factors, and amino acids via signaling through Akt, MAPK, and AMPK pathways (3,4). Recent studies found that the four related GTPases, RagA, RagB, RagC, and RagD, interact with raptor within the mTORC1 complex (1,2). These interactions are both necessary and sufficient for mTORC1 activation in response to amino acid signals (1,2). RagD is one of several genes differentially expressed in human melanoma cell lines and has been considered as a viable target for further diagnostic and therapeutic study (5).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nbA11D8.2.1; DKFZp761H171; FLJ44176; Rag D; Rag D protein; RagD; Ras related GTP binding D; Ras-related GTP binding D; Ras-related GTP-binding protein D; RRAGD\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H\nSENSITIVITY: Endogenous\nMW (kDa): 50\nSOURCE: Rabbit","brand":"CST","offers":[{"title":"Default Title","offer_id":46799514894505,"sku":"4470S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-4470s","provider":"Iright","version":"1.0","type":"link"}