{"product_id":"cst-4472s","title":"CST,  4472S, HPK1 Antibody","description":"Polyclonal Antibody for studying HPK1. Validated for Western Blotting,Immunoprecipitation. Highly specific and rigorously validated in-house, HPK1 Antibody (CST #4472) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nImmunoprecipitation: 1:50\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nHPK1 Antibody detects endogenous levels of total HPK1. This antibody does not cross-react with other members of the GCK family.\nSpecies Reactivity: Human, Mouse\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nPolyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to amino-terminal residues of human HPK1. Antibodies are purified by protein A and peptide affinity chromatography.\n\u003cb\u003eBackground\u003c\/b\u003e\nHematopoietic progenitor kinase 1 (HPK1) is a member of the germinal center kinases that is predominantly expressed in hematopoietic cells. HPK1 is a key component linking T or B cell receptors to SAPK\/JNK and IkappaB kinase pathways in lymphocytes (1-3). Through its proline-rich motif, HPK1 associates with multiple SH3 domain-containing adaptor proteins, including GRB2, Nck, Crk, SLP-76, and BLNK. Phosphorylation of Tyr379 by Syk is necessary for HPK1 association with BLNK and SLP-76, as well as for HPK1 activity (4,5). Caspase cleavage of HPK1 also modulates the biological function of HPK1 (3,6,7).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nHematopoietic progenitor kinase; hematopoietic progenitor kinase 1; HPK1; M4K1; MAP4K1; MAPK\/ERK kinase kinase kinase 1; MEK kinase kinase 1; MEKKK 1; Mitogen-activated protein kinase kinase kinase kinase 1\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M\nSENSITIVITY: Endogenous\nMW (kDa): 97\nSOURCE: Rabbit","brand":"CST","offers":[{"title":"Default Title","offer_id":46799515123881,"sku":"4472S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-4472s","provider":"Iright","version":"1.0","type":"link"}