{"product_id":"cst-4482s","title":"CST,  4482S, THEMIS Antibody","description":"Polyclonal Antibody for studying THEMIS. Validated for Western Blotting. Highly specific and rigorously validated in-house, THEMIS Antibody (CST #4482) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nTHEMIS Antibody recognizes endogenous levels of total THEMIS protein.\nSpecies Reactivity: Human\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nPolyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human THEMIS protein. Antibodies are purified by protein A and peptide affinity chromatography.\n\u003cb\u003eBackground\u003c\/b\u003e\nTHEMIS is a recently identified protein found to be critical for T cell development (1-5). It contains two amino-terminal globular domains, a nuclear localization signal, and a carboxy-terminal proline-rich sequence with homology to SH3 binding domains (2,3). THEMIS is detected in both the cytoplasm and the nucleus (2). It is expressed at low levels in mature T cells and during thymocyte development, with expression peaking in CD4 CD8 double positive cells (1-5). THEMIS is tyrosine phosphorylated downstream of TCR signaling and associates with the adaptor GRB2 and possibly other SH3 domain-containing proximal TCR signaling molecules (1-5). Mice lacking THEMIS have a defect in positive selection that results in decreased numbers of both single positive thymocytes and mature T cells (1-5).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nbA325O24.3; bA325O24.4; C6orf190; C6orf207; FLJ40584; GASP; GRB2-associated protein; MGC163388; Protein THEMIS; signaling phosphoprotein specific for T cells; SPOT; THEMIS; THMS1; thymocyte selection associated; thymocyte selection pathway associated; Thymocyte-expressed molecule involved in selection; TSEPA\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H\nSENSITIVITY: Endogenous\nMW (kDa): 74\nSOURCE: Rabbit","brand":"CST","offers":[{"title":"Default Title","offer_id":46799515975849,"sku":"4482S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-4482s","provider":"Iright","version":"1.0","type":"link"}