{"product_id":"cst-44947s","title":"CST,  44947S, CD14 (61D3) Mouse Monoclonal Antibody (PE-Cy7® Conjugate)","description":"Monoclonal Antibody for studying CD14. Validated for Flow Cytometry (Fixed\/Permeabilized),Flow Cytometry (Live). Highly specific and rigorously validated in-house, CD14 (61D3) Mouse Monoclonal Antibody (PE-Cy7\u003csup\u003e®\u003c\/sup\u003e Conjugate) (CST #44947) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nFlow Cytometry (Fixed\/Permeabilized): 1:20\nFlow Cytometry (Live): 1:20\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM NaH 2 PO 4 , 150 mM NaCl, 0.09% NaN 3 , 0.1% gelatin, pH7.2. This product is stable for 6 months when stored at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Flow Cytometry (Fixed\/Permeabilized), Flow Cytometry (Live)\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nCD14 (61D3) Mouse Monoclonal Antibody (PE-Cy7\nSpecies Reactivity: Human\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nThis monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.\n\u003cb\u003eBackground\u003c\/b\u003e\nCD14 is a leucine-rich repeat-containing pattern recognition receptor with expression largely restricted to the monocyte\/macrophage cell lineage (1). Research studies have shown that CD14 is a bacterial lipopolysaccharide (LPS) binding glycoprotein, expressed as either a GPI-linked membrane protein or a soluble plasma protein (2). LPS induces an upregulation of GPI-linked CD14 expression, which facilitates TLR4 signaling and macrophage activation in response to bacterial infection (3-5). The 61D3 antibody is widely used to identify cells of the monocyte\/macrophage lineage (6)\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nCD14; CD14 antigen; CD14 molecule; Monocyte differentiation antigen CD14; Monocyte differentiation antigen CD14, membrane-bound form; Monocyte differentiation antigen CD14, urinary form; Myeloid cell-specific leucine-rich glycoprotein\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H\nSENSITIVITY: Endogenous\nSource\/Isotype: Mouse IgG1","brand":"CST","offers":[{"title":"Default Title","offer_id":46800404938921,"sku":"44947S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-44947s","provider":"Iright","version":"1.0","type":"link"}