{"product_id":"cst-4508s","title":"CST,  4508S, Phospho-TAK1 (Thr184\/187) (90C7) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying TAK1 (Thr184\/Thr187) phosphate. Validated for Western Blotting,Simple Western™. Available in 3 sizes. Highly specific and rigorously validated in-house, Phospho-TAK1 (Thr184\/187) (90C7) Rabbit Monoclonal Antibody (CST #4508) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nSimple Western™: 1:10 - 1:50\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nPhospho-TAK1 (Thr184\/187) (90C7) Rabbit Monoclonal Antibody detects endogenous levels of TAK1 only when phosphorylated at threonine 184 and threonine 187. In some cell lysates, this antibody may cross-react with a 40 kDa band of unknown origin.\nSpecies Reactivity: Human\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a phosphopeptide corresponding to residues surrounding Thr184 and Thr187 of human TAK1.\n\u003cb\u003eBackground\u003c\/b\u003e\nTAK1 is a mitogen-activated protein kinase kinase kinase that can be activated by TGF-β, bone morphogenetic protein, and other cytokines, including IL-1 (1,2). activation of TAK1 requires association with TAK1 binding protein 1 (TAB1), which triggers phosphorylation of TAK1 (3,4). Another adaptor protein, TAB2, links TAK1 with TRAF6 and mediates TAK1 activation upon IL-1 stimulation (5). Once activated, TAK1 phosphorylates MAPK kinases MKK4 and MKK3\/6, which activate p38 MAPK and JNK, respectively. In addition, TAK1 activates the NF-κB pathway by interacting with TRAF6 and phosphorylating the NF-κB inducing kinase (NIK) (2).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nCSCF; FMD2; M3K7; MAP3K7; MEKK7; Mitogen-activated protein kinase kinase kinase 7; TAK1; TGF-beta activated kinase 1; TGF-beta-activated kinase 1; TGF1a; Transforming growth factor-beta-activated kinase 1\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H\nSENSITIVITY: Endogenous\nMW (kDa): 82\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46799517548713,"sku":"4508S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-4508s","provider":"Iright","version":"1.0","type":"link"}