{"product_id":"cst-4514s","title":"CST,  4514S, Phospho-SEK1\/MKK4 (Ser257) (C36C11) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying MKK4 (Ser257) phosphate. Validated for Western Blotting,Flow Cytometry (Fixed\/Permeabilized). Available in 2 sizes. Highly specific and rigorously validated in-house, Phospho-SEK1\/MKK4 (Ser257) (C36C11) Rabbit Monoclonal Antibody (CST #4514) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nFlow Cytometry (Fixed\/Permeabilized): 1:400\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 75450 .\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Flow Cytometry (Fixed\/Permeabilized)\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nPhospho-SEK1\/MKK4 (Ser257) (C36C11) Rabbit Monoclonal Antibody detects endogenous levels of SEK1\/MKK4 phosphorylated at Ser257 .\nSpecies Reactivity: Human, Mouse, Rat, Monkey\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser257\/Thr261 of human SEK1\/MKK4.\n\u003cb\u003eBackground\u003c\/b\u003e\nSAPK\/Erk kinase (SEK1), also known as MKK4 or Jun kinase kinase (JNKK), activates the MAP kinase homologues SAPK and JNK in response to various cellular stresses and inflammatory cytokines (1-3). Activation of SEK1 occurs through MEKK phosphorylation of serine and threonine residues at positions 257 and 261, respectively. Like MEK, SEK is a dual-specificity protein kinase that phosphorylates SAPK\/JNK at a conserved T*PY* site in its activation loop (4). Phosphorylation by Akt at Ser80 inhibits SEK1 and suppresses stress-activated signal transduction (5).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nc-Jun N-terminal kinase kinase 1; Dual specificity mitogen-activated protein kinase kinase 4; JNK activating kinase 1; JNK-activated kinase 1; JNK-activating kinase 1; JNKK; JNKK1; MAP kinase kinase 4; MAP2K4; MAPK\/ERK kinase 4; MAPKK 4; MAPKK4; MEK 4; MEK4; mitogen-activated protein kinase kinase 4; MKK4; MP2K4; PRKMK4; SAPK kinase 1; SAPK\/ERK kinase 1; SAPKK-1; SAPKK1; SEK1; SERK1; SKK1; Stress-activated protein kinase kinase 1\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R Mk\nSENSITIVITY: Endogenous\nMW (kDa): 44\nSource\/Isotype: Rabbit","brand":"CST","offers":[{"title":"Default Title","offer_id":46799518073001,"sku":"4514S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-4514s","provider":"Iright","version":"1.0","type":"link"}