{"product_id":"cst-45153t","title":"CST,  45153T, Phospho-Ezh2 (Thr345) (E7T7K) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying EZH2 (Thr345) phosphate. Validated for WB,IP,ChIP,ChIP,C\u0026amp;R. Available in 2 sizes. Highly specific and rigorously validated in-house, Phospho-Ezh2 (Thr345) (E7T7K) Rabbit Monoclonal Antibody (CST #45153) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nFor optimal ChIP and ChIP-seq results, use 10 μL of antibody and 10 μg of chromatin (approximately 4 x 10 6 cells) per IP. This antibody has been validated using SimpleChIP ® Enzymatic Chromatin IP Kits. The CUT\u0026amp;RUN dilution was determined using CUT\u0026amp;RUN Assay Kit # 86652 .\nWestern Blotting: 1:1000\nImmunoprecipitation: 1:50\nChromatin IP: 1:50\nChromatin IP-seq: 1:50\nCUT\u0026amp;RUN: 1:50\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation, Chromatin IP, Chromatin IP-seq, CUT\u0026amp;RUN\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nPhospho-Ezh2 (Thr345) (E7T7K) Rabbit Monoclonal Antibody recognizes endogenous levels of total Ezh2 protein only when phosphorylated at Thr345. This antibody may detect a band of unknown origin around 135 kDa.\nSpecies Reactivity: Human, Mouse, Rat, Monkey\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr345 of human Ezh2 protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nThe polycomb group (PcG) proteins are involved in maintaining the silenced state of several developmentally regulated genes and contribute to the maintenance of cell identity, cell cycle regulation, and oncogenesis (1,2). Enhancer of zeste homolog 2 (Ezh2), a member of this large protein family, contains four conserved regions, including domain I, domain II, and a cysteine-rich amino acid stretch that precedes the carboxy-terminal SET domain (3). The SET domain has been linked with histone methyltransferase (HMTase) activity. Moreover, mammalian Ezh2 is a member of a histone deacetylase complex that functions in gene silencing, acting at the level of chromatin structure (4). Ezh2 complexes methylate histone H3 at Lys9 and 27 , which is thought to be involved in targeting transcriptional regulators to specific loci (5). Ezh2 is deregulated in various tumor types, and its role, both as a primary effector and as a mediator of tumorigenesis, has become a subject of increased interest (6). Ezh2 is phosphorylated on Thr345 by cyclin-dependent kinase 1 (CDK1), which increases targeting of Ezh2 to its target loci and promotes methylation of H3 at Lys27 (7,8). This activated form of Ezh2 may deposit initial H3K27me3 marks onto newly synthesized daughter strands of DNA during replication (9). In addition, phosphorylation of Thr345 affects Ezh2 stability by promoting its ubiquitination and subsequent degradation (9).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nenhancer of zeste 2; enhancer of zeste 2 polycomb repressive complex 2 subunit; Enhancer of zeste homolog 2; enhancer of zeste homolog 2 (Drosophila); ENX-1; ENX1; EZH1; EZH2; EZH2b; Histone-lysine N-methyltransferase EZH2; KMT6; KMT6A; Lysine N-methyltransferase 6; MGC9169; WVS; WVS2\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R Mk\nSENSITIVITY: Endogenous\nMW (kDa): 98\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46799518302377,"sku":"45153T","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-45153t","provider":"Iright","version":"1.0","type":"link"}