{"product_id":"cst-45381sf","title":"CST,  45381SF, ERG (A7L1G) Rabbit Monoclonal Antibody (BSA and Azide Free)","description":"Monoclonal Antibody for studying ERG. Validated for WB,IHC,IF,F. Highly specific and rigorously validated in-house, ERG (A7L1G) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #45381) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nThis product is the carrier free version of product #97249. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT\u0026amp;RUN or CUT\u0026amp;Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions\/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity.\n\u003cb\u003eFormulation\u003c\/b\u003e\nSupplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 97249\n\u003cb\u003eStorage\u003c\/b\u003e\nStore at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze\/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance.\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nERG (A7L1G) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total ERG protein. Based on sequence identity, this antibody should detect isoforms ERG1, ERG2 and ERG3. This antibody does not cross-react with Fli1. Non-specific staining was observed in human stomach epithelium by immunohistochemistry. This antibody is not recommended for immunohistochemical analysis of mouse tissues.\nSpecies Reactivity: Human, Mouse\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human ERG protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nETS-related gene (ERG) is a member of the E-26 transformation-specific (ETS) family of sequence-specific DNA-binding transcription factors (1). ERG plays important and highly conserved roles in vertebrate development. Early in embryonic development, ERG is highly expressed in the embryonic mesoderm and endothelium, where it plays a critical role in the formation of the vascular system, urogenital tract and bone development (2,3). Later in embryonic development, ERG functions to regulate the pluripotency of hematopoietic stem cells, endothelial cell homeostasis and angiogenesis (2,4-7). ERG expression is not restricted to development. In adult mouse, ERG is normally expressed in endothelial tissues, including adrenal, cartilage, heart, spleen, lymphatic endothelial and eosinophil cells (8). However, deregulation of ERG activity, often resulting from chromosomal rearrangements, has been implicated and linked to poor prognosis in a number of different cancers. Chromosomal translocations generating EWS\/ERG chimeric proteins comprised of the amino-terminal transactivation domain of Ewing's sarcoma breakpoint region 1 (EWS) and the carboxy-terminal ETS domain of ERG have been identified in 5-10% of Ewing's sarcoma, an aggressive bone and soft tissue tumor (9). Chromosomal translocations between ERG and TLS\/FUS or ERG and ELF4 have been implicated in acute myeloid leukemia (10, 11). Over-expression of ERG, resulting from gene fusion with the androgen-driven promoter of the TMPRSS2 gene, has been identified as a key driver of metastasis and marker for poor prognosis in prostate cancer (12).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nERG; erg-3; ERG, ETS transcription factor; erythroblast transformation-specific transcription factor ERG variant 10; ETS transcription factor ERG; ets-related; FUS\/ERG fusion protein; p55; TMPRSS2-ERG prostate cancer specific; TMPRSS2\/ERG fusion; Transcriptional regulator ERG; transcriptional regulator ERG (transforming protein ERG); Transforming protein ERG; v-ets avian erythroblastosis virus E26 oncogene homolog; v-ets avian erythroblastosis virus E26 oncogene related; v-ets erythroblastosis virus E26 oncogene homolog; v-ets erythroblastosis virus E26 oncogene homolog (avian); v-ets erythroblastosis virus E26 oncogene like\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M\nSENSITIVITY: Endogenous\nMW (kDa): 54\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46799519547561,"sku":"45381SF","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-45381sf","provider":"Iright","version":"1.0","type":"link"}