{"product_id":"cst-45469t","title":"CST,  45469T, CD58 (F1C9B) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying CD58. Validated for Western Blotting,Immunohistochemistry (Paraffin). Available in 2 sizes. Highly specific and rigorously validated in-house, CD58 (F1C9B) Rabbit Monoclonal Antibody (CST #45469) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nImmunohistochemistry (Paraffin): 1:50 - 1:200\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 90094 .\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunohistochemistry (Paraffin)\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nCD58 (F1C9B) Rabbit Monoclonal Antibody recognizes endogenous levels of total CD58 protein.\nSpecies Reactivity: Human\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with recombinant protein specific to the extracellular domain of human CD58 protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nCD58, also known as lymphocyte function-associated antigen 3 (LFA-3), is a ubiquitously expressed glycoprotein anchored to the plasma membrane through a transmembrane domain or a GPI linkage (1). Research studies have demonstrated that CD58 plays a critical role in immune synapse formation by functioning as a co-stimulatory adhesion molecule upon engagement of the CD2 antigen on the surface of T cells and NK cells. As such, the CD58-CD2 axis facilitates the execution of immune effector cell functions, including activation, proliferation, and cytotoxicity (2-4). In the context of cancer, recent studies have pointed to the CD58-CD2 axis as a stimulatory immune checkpoint, and lesions to this axis compromise anti-tumor immune responses (5,6).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nAg3; CD58; CD58 antigen, (lymphocyte function-associated antigen 3); CD58 molecule; LFA-3; LFA3; Lymphocyte function-associated antigen 3; Surface glycoprotein LFA-3\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H\nSENSITIVITY: Endogenous\nMW (kDa): 55-70\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46799520596137,"sku":"45469T","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-45469t","provider":"Iright","version":"1.0","type":"link"}