{"product_id":"cst-45829v1","title":"CST,  45829V1, FastScan™ Total Cyclin E1 ELISA Kit","description":"FastScan ELISA Kit for studying Cyclin E1 in the research area.\n\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: ELISA+\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nThe FastScan™ Total Cyclin E1 ELISA Kit detects endogenous levels of cyclin E1, as shown in Figure 1. This kit detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species.\nSpecies Reactivity: Human\n\u003cb\u003eBackground\u003c\/b\u003e\nCyclin E1 and cyclin E2 can associate with and activate CDK2 (1). Upon DNA damage, upregulation\/activation of the CDK inhibitors p21 Waf1\/Cip1 and p27 Kip1 prevent cyclin E\/CDK2 activation, resulting in G1\/S arrest. When conditions are favorable for cell cycle progression, cyclin D\/CDK4\/6 phosphorylates Rb and is thought to reduce the activity of p21 Waf1\/Cip1 and p27 Kip1, allowing subsequent activation of cyclin E\/CDK2 (1,2). Cyclin E\/CDK2 further phosphorylates Rb to allow progression into S-phase, where cyclin E\/CDK2 is thought to phosphorylate and activate multiple proteins involved in DNA synthesis (2,3). Turnover of cyclin E is largely controlled by phosphorylation that results in SCFFbw7-mediated ubiquitination and proteasome-dependent degradation (4,5). Cyclin E1 is phosphorylated at multiple sites including Thr62, Ser88, Ser72, Thr380, and Ser384, and is controlled by at least two kinases, CDK2 and GSK-3 (6,7).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nCCNE; CCNE1; cyclin E1; cyclin Es; cyclin Et; G1\/S-specific cyclin-E1; pCCNE1\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H","brand":"CST","offers":[{"title":"Default Title","offer_id":46800604332201,"sku":"45829V1","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-45829v1","provider":"Iright","version":"1.0","type":"link"}