{"product_id":"cst-45964s","title":"CST,  45964S, CNPase (D83E10) Mouse Chimeric Monoclonal Antibody","description":"Monoclonal Antibody for studying CNPase. Validated for Immunofluorescence (Frozen). Available in 2 sizes. Highly specific and rigorously validated in-house, CNPase (D83E10) Mouse Chimeric Monoclonal Antibody (CST #45964) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nImmunofluorescence (Frozen): 1:50\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Immunofluorescence (Frozen)\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nCNPase (D83E10) Mouse Chimeric Monoclonal Antibody recognizes endogenous levels of CNPase protein.\nSpecies Reactivity: Human, Mouse, Rat\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nThis recombinant chimeric antibody is engineered from CNPase (D83E10) XP  Rabbit mAb #5664 according to animal-free protocols. The chimeric antibody retains its antigen-binding Fab regions from the original rabbit monoclonal antibody but contains a mouse-derived Fc domain. When multiplexing, Fc-directed rabbit secondaries are required to detect rabbit-host primary antibodies. The parent antibody, CNPase (D83E10) XP  Rabbit mAb #5664, is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val81 of human CNPase protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nCNPase (2', 3'-cyclic nucleotide 3'-phosphodiesterase) catalyzes the hydrolysis of 2', 3'-cyclic nucleotides to produce 2'-nucleotides. The molecular function and native substrate of this nucleotide phosphodiesterase remains under investigation (1). High CNPase expression is seen in oligodendrocytes and Schwann cells as CNPase accounts for roughly 4% of the total myelin protein in the central nervous system (2). CNPase binds to tubulin heterodimers and plays a role in tubulin polymerization, and oligodendrocyte process outgrowth (3). Typical myelination is seen in CNPase knock-out mice, but they suffer severe neurodegeneration from axonal loss and oligodendrocytes display abnormal paranodal loop structure prior to axonal degeneration. Paranodal loops typically contact the axolemma in axon-glial signaling; neurodegeneration in CNPase knock-out mice is a secondary consequence of impaired cell-cell communication (4).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\n2', 3' cyclic nucleotide 3' phosphohydrolase; 2',3'-cyclic nucleotide 3' phosphodiesterase; 2',3'-cyclic-nucleotide 3'-phosphodiesterase; CN37; CNP; CNP1; CNPase\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R\nSENSITIVITY: Endogenous\nSource\/Isotype: Mouse chimeric IgG2a","brand":"CST","offers":[{"title":"Default Title","offer_id":46800294838441,"sku":"45964S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-45964s","provider":"Iright","version":"1.0","type":"link"}